Rabbit polyclonal NCK1 antibody (A0999)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NCK1 antibody (A0999)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NCK1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

NCK1 antibody,MGC12668 antibody,NCK antibody,NCKalpha antibody
Cytoplasmic protein NCK1 antibody|Melanoma Nck protein antibody|MGC12668 antibody|NCK 1 antibody|NCK adaptor protein 1 antibody|NCK alpha antibody|NCK antibody|NCK tyrosine kinase antibody|Nck-1 antibody|NCK1 antibody|NCK1_HUMAN antibody|NCKalpha antibody|Non catalytic region of tyrosine kinase antibody|SH2/SH3 adaptor protein NCK alpha antibody|SH2/SH3 adaptor protein NCK-alpha antibody|Anti-Nck antibody [Y531] (ab32120)

SCBT cat No: sc-136232|sc-20026|sc-290|sc-166425|sc-365802|sc-20020|

Name

Rabbit polyclonal NCK1 antibody

Catalogue No.

A0999

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NCK1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 43kDa/Observed: Refer to Figures

Formulation

NCK1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NCK1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NCK1 antibody,MGC12668 antibody,NCK antibody,NCKalpha antibody

Database links

Human UniProt/Swiss-Prot:P16333

Protein names

NCK1,MGC12668,NCK,NCKalpha

Protein function

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Protein sequence and domain

Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the SH2 domains do not seeem to be involved.Contains 1 SH2 domain.; Contains 3 SH3 domains.

Protein post-translational modifications

Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated by activated PDGFRB.

Protein cellular localization

Cytoplasm. Endoplasmic reticulum. Nucleus. Note: Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.

Background

NCK1 (also known as NCK or NCKalpha) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKbeta), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

Research area

All research areas>Signaling Intermediates>NCK
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize NCK1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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