Rabbit polyclonal NEDD8 antibody (A1163)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit polyclonal NEDD8 antibody (A1163)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:100 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NEDD8 antibody.

Alternative names:

Check alternative names for the antibody

Expand

NEDD8 antibody,FLJ43224 antibody,MGC104393 antibody,MGC125896 antibody,MGC125897 antibody,Nedd-8 antibody
FLJ43224 antibody|MGC104393 antibody|MGC125896 antibody|MGC125897 antibody|NED8 antibody|NEDD 8 antibody|NEDD-8 antibody|Nedd8 antibody|NEDD8_HUMAN antibody|Neddylin antibody|Neural precursor cell expressed developmentally down regulated 8 antibody|Neural precursor cell expressed developmentally down regulated gene 8 antibody|Neural precursor cell expressed developmentally down-regulated protein 8 antibody|Rub1 antibody|Ubiquitin like protein Nedd 8 antibody|Ubiquitin like protein Nedd8 antibody|Ubiquitin-like protein Nedd8 antibody|Anti-NEDD8 antibody [Y297] (ab81264)

SCBT cat No: sc-30077|sc-373741|sc-5480|

Name

Rabbit polyclonal NEDD8 antibody

Catalogue No.

A1163

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NEDD8

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 9kDa/Observed: Refer to Figures

Formulation

NEDD8 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NEDD8 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NEDD8 antibody,FLJ43224 antibody,MGC104393 antibody,MGC125896 antibody,MGC125897 antibody,Nedd-8 antibody

Database links

Human UniProt/Swiss-Prot:Q15843

Protein names

NEDD8,FLJ43224,MGC104393,MGC125896,MGC125897,Nedd-8

Protein function

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Protein tissue specificity

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

Protein sequence and domain

Belongs to the ubiquitin family.

Protein post-translational modifications

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

Protein cellular localization

Nucleus . Note: Mainly nuclear.

Background

Neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8), also known as Rub1 (related to ubiquitin 1) in plants and yeast, is a member of the ubiquitin-like protein family (1,2). The covalent attachment of NEDD8 to target proteins, termed neddylation, is a reversible, multi-step process analogous to ubiquitination. NEDD8 is first synthesized in a precursor form with a carboxy-terminal extension peptide that is removed by either the UCH-L3 or NEDP1/DEN1 hydrolase protein to yield a mature NEDD8 protein (3,4). Mature NEDD8 is then covalently linked to target proteins via the carboxy-terminal glycine residue in a reaction catalyzed by the APP-BP1/Uba3 heterodimer complex and Ubc12 as the E1- and E2-like enzymes, respectively (5). An E3 ligase protein, Roc1/Rbx1, is also required for neddylation of the cullin proteins (6). Protein de-neddylation is catalyzed by a number of enzymes in the cell, including a “ubiquitin-specific” protease USP21, the NEDP1/DEN1 hydrolase and the COP9/signalosome (CSN) (7,8,9). In contrast to the ubiquitin pathway, the NEDD8 modification system acts on only a few substrates and does not appear to target proteins for degradation. Neddylation of cullin proteins activates the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex by promoting complex formation and enhancing the recruitment of the E2-ubiquitin intermediate (10). While NEDD8 modification of VHL is not required for ubiquitination of HIF1-alpha, it is required for fibronectin matrix assembly (11). Mdm2-dependent neddylation of p53 inhibits its transcriptional activity (12).

Research area

All research areas>Synthesis and Degradation>NEDD
(View all antibody categories related to Synthesis and Degradation)

Note

AntibodyPlus can customize NEDD8 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!