Rabbit polyclonal NEFL antibody (A0257)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NEFL antibody (A0257)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NEFL antibody.

Alternative names:

Check alternative names for the antibody

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NEFL antibody,CMT1F antibody,CMT2E antibody, NF L antibody,NFL antibody,68 kDa neurofilament protein antibody
68 kDa neurofilament protein antibody|68kDa neurofilament protein antibody|CMT1F antibody|CMT2E antibody|FLJ53642 antibody|Light molecular weight neurofilament protein antibody|NEFL antibody|Neurofilament light antibody|Neurofilament light polypeptide 68kDa antibody|Neurofilament light polypeptide antibody|Neurofilament protein, light chain antibody|Neurofilament subunit NF L antibody|Neurofilament triplet L protein antibody|NF-L antibody|NF68 antibody|NFL antibody|NFL_HUMAN antibody|Anti-68kDa Neurofilament antibody (ab72997)

SCBT cat No: sc-71678|sc-20012|sc-12980|sc-58559|sc-390732|sc-25652|sc-12966|sc-58560|sc-52350|

Name

Rabbit polyclonal NEFL antibody

Catalogue No.

A0257

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Protein of human NEFL

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 62kDa/Observed: Refer to Figures

Formulation

NEFL antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NEFL antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NEFL antibody,CMT1F antibody,CMT2E antibody, NF L antibody,NFL antibody,68 kDa neurofilament protein antibody

Database links

Human UniProt/Swiss-Prot:P07196

Protein names

NEFL,CMT1F,CMT2E, NF L,NFL,68 kDa neurofilament protein

Protein function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Protein sequence and domain

The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.Belongs to the intermediate filament family.

Protein post-translational modifications

O-glycosylated.; Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.; Ubiquitinated in the presence of TRIM2 and UBE2D1.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central alpha-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinson’s disease (in Lewy bodies along with alpha-synuclein), Alzheimer’s disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

Research area

All research areas>Neurobiology>NF
(View all antibody categories related to Neurobiology)

Note

AntibodyPlus can customize NEFL antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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