Rabbit polyclonal NFATC1 antibody (A1539)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NFATC1 antibody (A1539)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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MGC138448 antibody, NF-ATC antibody, NFAT2 antibody, NFATc antibody
cytoplasmic 1 antibody|MGC138448 antibody|NF ATc antibody|NF ATc1 antibody|NF-ATc antibody|NF-ATc1 antibody|NF-ATc1.2 antibody|NFAC1_HUMAN antibody|NFAT 2 antibody|NFAT transcription complex cytosolic component antibody|NFATC 1 antibody|NFATc antibody|NFATc1 antibody|Nuclear factor of activated T cells cytoplasmic 1 antibody|Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody|Nuclear factor of activated T cells cytosolic component 1 antibody|nuclear factor of activated T-cells ‘c’ antibody|Nuclear factor of activated T-cells antibody|Anti-NFAT2 antibody [7A6] – ChIP Grade (ab2796)

SCBT cat No: sc-32979|

Name

Rabbit polyclonal NFATC1 antibody

Catalogue No.

A1539

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NFATC1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 78kDa;101kDa/Observed: Refer to Figures

Formulation

NFATC1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NFATC1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

MGC138448 antibody, NF-ATC antibody, NFAT2 antibody, NFATc antibody

Database links

Human UniProt/Swiss-Prot:O95644

Protein names

MGC138448, NF-ATC, NFAT2, NFATc

Protein function

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells . Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).

Protein tissue specificity

Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.

Protein sequence and domain

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.; The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.; Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.Contains 1 RHD (Rel-like) domain.

Protein post-translational modifications

Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1.

Protein cellular localization

Cytoplasm . Nucleus . Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription . Nuclear translocation of NFATC1 is enhanced in the presence of TNFSF11. Nuclear translocation is decreased in the presence of FBN1 which can bind and sequester TNFSF11 (By similarity).

Background

The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1,2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3,4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5).

Research area

All research areas>Transcription Regulators>NFAT
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize NFATC1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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