Rabbit polyclonal NFKBIA antibody (A1187)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NFKBIA antibody (A1187)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

NFKBIA antibody,IKBA antibody,MAD-3 antibody,NFKBI antibody
I kappa B alpha antibody|I-kappa-B-alpha antibody|IkappaBalpha antibody|IkB-alpha antibody|IKBA antibody|IKBA_HUMAN antibody|IKBalpha antibody|MAD 3 antibody|MAD3 antibody|Major histocompatibility complex enhancer-binding protein MAD3 antibody|NF kappa B inhibitor alpha antibody|NF-kappa-B inhibitor alpha antibody|NFKBI antibody|NFKBIA antibody|Nuclear factor of kappa light chain gene enhancer in B cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody|Anti-IKB alpha antibody [E130] (ab32518)

SCBT cat No: sc-71291|sc-52900|sc-23459|sc-23460|sc-25105|sc-71525|sc-52936|sc-23461|sc-56196|sc-56217|sc-52953|sc-71965|

Name

Rabbit polyclonal NFKBIA antibody

Catalogue No.

A1187

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NFKBIA

Host

Rabbit

Applications

WB, IHC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 39kDa/Observed: Refer to Figures

Formulation

NFKBIA antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NFKBIA antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NFKBIA antibody,IKBA antibody,MAD-3 antibody,NFKBI antibody

Database links

Human UniProt/Swiss-Prot:P25963

Protein names

NFKBIA,IKBA,MAD-3,NFKBI

Protein function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Protein sequence and domain

Belongs to the NF-kappa-B inhibitor family.; Contains 5 ANK repeats.

Protein post-translational modifications

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.; Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.; Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.; Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

Protein cellular localization

Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

Background

The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkappaB proteins (1-3). Activation occurs via phosphorylation of IkappaBalpha at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-kappaB (3-7). IkappaBalpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IkappaB at these activating sites have been identified (8).

Research area

All research areas>Transcription Regulators>I κ B
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize NFKBIA antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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