Rabbit polyclonal NOL3 antibody (A6319)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NOL3 antibody (A6319)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:10 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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ARC antibody, FCM antibody, MYP antibody, NOP antibody, NOP30 antibody
Apoptosis repressor with CARD antibody|ARC antibody|Muscle enriched cytoplasmic protein antibody|Muscle-enriched cytoplasmic protein antibody|MYC antibody|MYP antibody|Nol3 antibody|NOL3_HUMAN antibody|NOP antibody|Nop30 antibody|Nucleolar protein 3 (apoptosis repressor with CARD domain) antibody|Nucleolar protein 3 antibody|Nucleolar protein of 30 kDa antibody|Anti-Apoptosis repressor with CARD antibody (ab126238)

SCBT cat No: sc-390949|sc-166461|sc-17839|sc-6382|sc-55475|sc-374177|

Name

Rabbit polyclonal NOL3 antibody

Catalogue No.

A6319

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NOL3

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:10 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 24kDa/Observed: Refer to Figures

Formulation

NOL3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NOL3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ARC antibody, FCM antibody, MYP antibody, NOP antibody, NOP30 antibody

Database links

Human UniProt/Swiss-Prot:O60936

Protein names

ARC, FCM, MYP, NOP, NOP30

Protein function

Isoform 1: May be involved in RNA splicing.; Isoform 2: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation (By similarity). Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors . Inhibits calcium-mediated cell death by functionning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis . Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation (By similarity). Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner (By similarity). Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I (By similarity). Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia (By similarity). Inhibits too myoblast differentiation through caspase inhibition (By similarity).

Protein tissue specificity

Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.

Protein sequence and domain

CARD is critical for both extrinsic and intrinsic apoptotic pathways (By similarity). CARD domain mediates a protective effect against myocardial ischemia/reperfusion, oxidative stress and TNF-induced necrosis (By similarity). The calcium binding domain plays a protective role in calcium-mediated cell death .Contains 1 CARD domain.

Protein post-translational modifications

Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mithochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin; doesn’t inhibit the association between FADD and CASP8 and the consequent apoptosis.; Polyubiquitinated by MDM2; promoting proteasomal-dependent degradation in response to apoptotic stimuli.

Protein cellular localization

Isoform 1: Nucleus, nucleolus . Note: The SR-rich C-terminus mediates nuclear localization.; Isoform 3: Cytoplasm .; Isoform 2: Cytoplasm . Mitochondrion . Sarcoplasmic reticulum . Membrane ; Lipid-anchor . Note: Phosphorylation at Thr-149 results in translocation to mithochondria. Colocalized with mitochondria in response to oxidative stress.

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene.

Research area

All research areas>Tumor Suppressor/Apoptosis>Apoptosis Repressor with CARD
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize NOL3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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