Rabbit polyclonal NOS3 antibody (A1548)


Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NOS3 antibody (A1548)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NOS3 antibody.

Alternative names:

Check alternative names for the antibody


eNOS antibody, ECNOS antibody
cNOS antibody|Constitutive NOS antibody|EC NOS antibody|EC-NOS antibody|ecNOS antibody|Endothelial nitric oxidase synthase antibody|Endothelial nitric oxide synthase antibody|Endothelial nitric oxide synthase 3 antibody|Endothelial NOS antibody|eNOS antibody|Nitric oxide synthase 3 (endothelial cell) antibody|Nitric oxide synthase 3 antibody|Nitric oxide synthase 3 endothelial cell antibody|Nitric oxide synthase endothelial antibody|Nitric oxide synthase, endothelial antibody|NOS 3 antibody|NOS III antibody|NOS type III antibody|NOS3 antibody|NOS3_HUMAN antibody|NOSIII antibody|Anti-eNOS antibody [M221] (ab76198)

SCBT cat No: sc-376751|sc-136977|sc-654|sc-376542|sc-8311|sc-653|sc-137118|sc-46423|sc-365363|sc-137111|sc-67003|sc-137117|sc-373954|


Rabbit polyclonal NOS3 antibody

Catalogue No.



Human, Mouse, Rat


Recombinant protein of human NOS3





Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200







Molecular weight

Predicted: 133kDa/Observed: Refer to Figures


NOS3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.


NOS3 antibody was purified using affinity purification.


Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

eNOS antibody, ECNOS antibody

Database links

Human UniProt/Swiss-Prot:P29474

Protein names


Protein function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.; Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Protein tissue specificity

Platelets, placenta, liver and kidney.

Protein sequence and domain

Belongs to the NOS family.; Contains 1 FAD-binding FR-type domain.; Contains 1 flavodoxin-like domain.

Protein post-translational modifications

Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Protein cellular localization

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.


Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).

Research area

All research areas>Synthesis and Degradation>NOS
(View all antibody categories related to Synthesis and Degradation)


AntibodyPlus can customize NOS3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody


ABclonal Inc.


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