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Rabbit polyclonal NOTCH1 antibody (A7636)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000 IHC 1:100 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal NOTCH1 antibody.
Check alternative names for the antibodyExpand
hN1 antibody, AOS5 antibody, TAN1 antibody, AOVD1 antibody
hN1 antibody|Neurogenic locus Notch homolog protein 1 antibody|NICD antibody|NOTC1_HUMAN antibody|Notch 1 antibody|Notch 1 intracellular domain antibody|Notch homolog 1 translocation associated (Drosophila) antibody|notch1 antibody|TAN1 antibody|Translocation-associated notch protein TAN-1 antibody|Anti-activated Notch1 antibody (ab8925)
SCBT cat No: sc-376403|sc-373891|sc-6014|sc-373944|sc-23301|sc-9170|sc-23299|sc-6015|sc-32745|
Rabbit polyclonal NOTCH1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
A synthetic peptide of human NOTCH1
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 273kDa/Observed: Refer to Figures
NOTCH1 antibody was tube-contained.
NOTCH1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
hN1 antibody, AOS5 antibody, TAN1 antibody, AOVD1 antibody
|Database links|| |
|Protein names|| |
hN1, AOS5, TAN1, AOVD1
|Protein function|| |
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
|Protein tissue specificity|| |
In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
|Protein sequence and domain|| |
Belongs to the NOTCH family.; Contains 6 ANK repeats.; Contains 36 EGF-like domains.; Contains 3 LNR (Lin/Notch) repeats.
|Protein post-translational modifications|| |
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by ADAM17 to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). Following endocytosis, this fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.; Phosphorylated.; O-glycosylated on the EGF-like domains . Contains both O-linked fucose and O-linked glucose in the EGF-like domains 11, 12 and 13, which are interacting with the residues on DLL4 (By similarity). O-linked glycosylation by GALNT11 is involved in determination of left/right symmetry: glycosylation promotes activation of NOTCH1, possibly by promoting cleavage by ADAM17, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO) .; Ubiquitinated; undergoes ‘Lys-29’-linked polyubiquitination catalyzed by ITCH. Monoubiquitination at Lys-1759 is required for activation by gamma-secretase cleavage, it promotes interaction with AAK1, which stabilizes it. Deubiquitination by EIF3F is necessary for nuclear import of activated Notch.; Hydroxylated at Asn-1955 by HIF1AN. Hydroxylated at Asn-2022 by HIF1AN (By similarity). Hydroxylation reduces affinity for HI1AN and may thus indirectly modulate negative regulation of NICD (By similarity).
|Protein cellular localization|| |
Cell membrane ; Single-pass type I membrane protein .; Notch 1 intracellular domain: Nucleus . Note: Following proteolytical processing NICD is translocated to the nucleus.
This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
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|Product type|| |
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