Rabbit polyclonal NOTCH2 antibody (A0560)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NOTCH2 antibody (A0560)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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hN2 antibody, AGS2 antibody, HJCYS antibody
AGS2 antibody|Aicardi-Goutieres syndrome 2 protein antibody|Deleted in lymphocytic leukemia 8 antibody|DLEU8 antibody|FLJ11712 antibody|Ribonuclease H2 subunit B antibody|Ribonuclease HI subunit B antibody|RNase H2 subunit B antibody|Rnaseh2b antibody|RNH2B_HUMAN antibody|Anti-Notch2 antibody (ab8926)

SCBT cat No: sc-7423|

Name

Rabbit polyclonal NOTCH2 antibody

Catalogue No.

A0560

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human NOTCH2

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:100 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 265kDa/Observed: Refer to figures

Formulation

NOTCH2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NOTCH2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

hN2 antibody, AGS2 antibody, HJCYS antibody

Database links

Human UniProt/Swiss-Prot:Q04721

Protein names

hN2, AGS2, HJCYS

Protein function

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells .

Protein tissue specificity

Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.

Protein sequence and domain

Belongs to the NOTCH family.; Contains 6 ANK repeats.; Contains 35 EGF-like domains.; Contains 3 LNR (Lin/Notch) repeats.

Protein post-translational modifications

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).; Hydroxylated by HIF1AN.; Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.

Protein cellular localization

Notch 2 extracellular truncation: Cell membrane ; Single-pass type I membrane protein .; Notch 2 intracellular domain: Nucleus . Cytoplasm . Note: Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 .

Background

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Research area

All research areas>Membrane Receptors>Notch
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize NOTCH2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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