Rabbit polyclonal NOTCH3 antibody (A3115)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NOTCH3 antibody (A3115)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:200 – 1:1000 IHC 1:20 – 1:200 IF 1:20 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal NOTCH3 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CASIL antibody, CADASIL antibody
CADASIL antibody|CASIL antibody|NOTC3_HUMAN antibody|Notch 3 antibody|Notch 3 intracellular domain antibody|Notch homolog 3 antibody|Notch3 antibody|Anti-NOTCH3 antibody (ab23426)

SCBT cat No: sc-32347|

Name

Rabbit polyclonal NOTCH3 antibody

Catalogue No.

A3115

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human NOTCH3

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:200 – 1:1000
IHC 1:20 – 1:200
IF 1:20 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 244kDa/Observed: Refer to Figures

Formulation

NOTCH3 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NOTCH3 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CASIL antibody, CADASIL antibody

Database links

Human UniProt/Swiss-Prot:Q9UM47

Protein names

CASIL, CADASIL

Protein function

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).

Protein tissue specificity

Ubiquitously expressed in fetal and adult tissues.

Protein sequence and domain

Belongs to the NOTCH family.; Contains 5 ANK repeats.; Contains 34 EGF-like domains.; Contains 3 LNR (Lin/Notch) repeats.

Protein post-translational modifications

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).; Phosphorylated.; Hydroxylated by HIF1AN.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein.; Notch 3 intracellular domain: Nucleus. Note: Following proteolytical processing NICD is translocated to the nucleus.

Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).Notch3 is a member of the Notch family and is processed similar to Notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the Notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicate that Notch3 is overexpressed in many types of cancer (9-11).

Research area

All research areas>Membrane Receptors>Notch
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize NOTCH3 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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