No products in the cart.
Rabbit polyclonal NOTCH3 antibody (A3115)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:200 – 1:1000 IHC 1:20 – 1:200 IF 1:20 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal NOTCH3 antibody.
Check alternative names for the antibodyExpand
CASIL antibody, CADASIL antibody
CADASIL antibody|CASIL antibody|NOTC3_HUMAN antibody|Notch 3 antibody|Notch 3 intracellular domain antibody|Notch homolog 3 antibody|Notch3 antibody|Anti-NOTCH3 antibody (ab23426)
SCBT cat No: sc-32347|
Rabbit polyclonal NOTCH3 antibody
|Catalogue No.|| |
Human, Mouse, Rat
A synthetic peptide of human NOTCH3
WB, IHC, IF
|Recommended dilution|| |
WB 1:200 – 1:1000
|Molecular weight|| |
Predicted: 244kDa/Observed: Refer to Figures
NOTCH3 antibody was tube-contained.
NOTCH3 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
CASIL antibody, CADASIL antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
|Protein tissue specificity|| |
Ubiquitously expressed in fetal and adult tissues.
|Protein sequence and domain|| |
Belongs to the NOTCH family.; Contains 5 ANK repeats.; Contains 34 EGF-like domains.; Contains 3 LNR (Lin/Notch) repeats.
|Protein post-translational modifications|| |
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).; Phosphorylated.; Hydroxylated by HIF1AN.
|Protein cellular localization|| |
Cell membrane; Single-pass type I membrane protein.; Notch 3 intracellular domain: Nucleus. Note: Following proteolytical processing NICD is translocated to the nucleus.
Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4).Notch3 is a member of the Notch family and is processed similar to Notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the Notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicate that Notch3 is overexpressed in many types of cancer (9-11).
AntibodyPlus can customize NOTCH3 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
There are no reviews for this product yet.
By submitting a review, get following benefits:
1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!
There is no extra validation for this product yet.
Check other extra validated antibodies below: