Rabbit Polyclonal Nrf2 antibody (STJ96702)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Nrf2 antibody (STJ96702)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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NFE2L2 antibody, NRF2 antibody,|erythroid derived 2 antibody||Phospho anti-Nrf2 (S40) antibody [EP1809Y] (ab76026)
SCBT cat No: sc-81342|sc-365949|sc-722|sc-13032|sc-30915|

Name

Nrf2 Polyclonal Antibody

Catalogue No.

STJ96702

Reactivity

Human, Mouse, Rat

Specificity

Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.

Immunogen

Synthesized peptide derived from human Nrf2 around the non-acetylation site of K599

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

68 kDa

Formulation

Nrf2 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Nrf2 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Nuclear factor erythroid 2-related factor 2 antibody, NF-E2-related factor 2 antibody, NFE2-related factor 2 antibody, HEBP1 antibody, Nuclear factor, erythroid derived 2, like 2 antibody

Database links

Human UniProt/Swiss-Prot:Q16236;Mouse UniPort/Swiss-Prot: Q60795;Rat UniProt/Swiss-Port: O54968;Human Entrez Gene: 4780;Mouse Entrez Gene: 18024;Rat Entrez Gene: Rn.10867

Protein names

Nuclear factor erythroid 2-related factor 2 , NF-E2-related factor 2 , NFE2-related factor 2 , HEBP1 , Nuclear factor, erythroid derived 2, like 2

Protein function

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Protein tissue specificity

Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

Protein sequence and domain

Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus. / Belongs to the bZIP family. CNC subfamily. / Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. / Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. / Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane.

Protein cellular localization

Cytoplasm > cytosol / Nucleus

Research area

All research areas>Transcription Regulators>Nrf2
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Nrf2 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Human stomach tissue

1: Nrf2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: Nrf2 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Human uterus tissue

1: Nrf2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung tissue

1: Nrf2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue

1: Nrf2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: Nrf2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: Nrf2 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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