Rabbit polyclonal NUMA1 antibody (A0527)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal NUMA1 antibody (A0527)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:200 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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NUMA antibody
Centrophilin stabilizes mitotic spindle in mitotic cells antibody|NMP 22 antibody|Nuclear matrix protein 22 antibody|Nuclear mitotic apparatus protein 1 antibody|Nuclear mitotic apparatus protein antibody|NUMA 1 antibody|NUMA antibody|NuMA protein antibody|NUMA1 antibody|NUMA1_HUMAN antibody|SP H antigen antibody|SP-H antigen antibody|Structural nuclear protein antibody|Anti-NuMA antibody (ab36999)

SCBT cat No: sc-56325|sc-18557|sc-51164|sc-365532|sc-48773|sc-18555|

Name

Rabbit polyclonal NUMA1 antibody

Catalogue No.

A0527

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human NUMA1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:200 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 133kDa/Observed: Refer to Figures

Formulation

NUMA1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

NUMA1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

NUMA antibody

Database links

Human UniProt/Swiss-Prot:Q14980

Protein names

NUMA

Protein function

Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume . Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes . Also required for proper alignment of the mitotic spindle during asymmetric cell divisions .

Protein sequence and domain

The C-terminal tubulin-binding domain mediates direct binding to microtubules, independently of dynein and dynactin, and induces their bundling and stabilization.

Protein post-translational modifications

ADP-ribosylated by TNKS during mitosis.; Phosphorylated in the C-terminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; Ubiquitinated with ‘Lys-63’-linked polyubiquitin chains. Deubiquitination by the BRISC complex is important for the incorporation of NUMA1 into mitotic spindle poles and normal spindle pole function, probably by modulating interactions between NUMA1, dynein and importin-beta.

Protein cellular localization

Nucleus matrix . Chromosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Note: Resides in the nuclear matrix during interphase. Dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association; it remains there until the anaphase onset. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. CCSAP is essential for its centrosomal localization .; Isoform Numa-m: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Note: During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.; Isoform Numa-s: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Note: During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.

Background

The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).

Research area

All research areas>Cell Cycle Proteins>NuMA
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize NUMA1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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