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Rabbit polyclonal NUMA1 antibody (A0527)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:200 – 1:1000 IHC 1:50 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal NUMA1 antibody.
Check alternative names for the antibodyExpand
Centrophilin stabilizes mitotic spindle in mitotic cells antibody|NMP 22 antibody|Nuclear matrix protein 22 antibody|Nuclear mitotic apparatus protein 1 antibody|Nuclear mitotic apparatus protein antibody|NUMA 1 antibody|NUMA antibody|NuMA protein antibody|NUMA1 antibody|NUMA1_HUMAN antibody|SP H antigen antibody|SP-H antigen antibody|Structural nuclear protein antibody|Anti-NuMA antibody (ab36999)
SCBT cat No: sc-56325|sc-18557|sc-51164|sc-365532|sc-48773|sc-18555|
Rabbit polyclonal NUMA1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human NUMA1
|Recommended dilution|| |
WB 1:200 – 1:1000
|Molecular weight|| |
Predicted: 133kDa/Observed: Refer to Figures
NUMA1 antibody was tube-contained.
NUMA1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
|Protein names|| |
|Protein function|| |
Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume . Required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes . Also required for proper alignment of the mitotic spindle during asymmetric cell divisions .
|Protein sequence and domain|| |
The C-terminal tubulin-binding domain mediates direct binding to microtubules, independently of dynein and dynactin, and induces their bundling and stabilization.
|Protein post-translational modifications|| |
ADP-ribosylated by TNKS during mitosis.; Phosphorylated in the C-terminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; Ubiquitinated with ‘Lys-63’-linked polyubiquitin chains. Deubiquitination by the BRISC complex is important for the incorporation of NUMA1 into mitotic spindle poles and normal spindle pole function, probably by modulating interactions between NUMA1, dynein and importin-beta.
|Protein cellular localization|| |
Nucleus matrix . Chromosome . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Note: Resides in the nuclear matrix during interphase. Dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association; it remains there until the anaphase onset. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. CCSAP is essential for its centrosomal localization .; Isoform Numa-m: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Note: During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.; Isoform Numa-s: Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Note: During interphase, mainly clustered at the centrosomal region in the cytosol. After entry into mitosis, detected at mitotic spindle poles.
The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).
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|Product type|| |
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