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Rabbit polyclonal OGT antibody (A1990)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF,IP
WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200 IP 1:20 – 1:50
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
HRNT1 antibody, O-GLCNAC antibody
FLJ23071 antibody|GlcNAc transferase antibody|HRNT1 antibody|MGC22921 antibody|O GlcNAc antibody|O GlcNAc transferase p110 subunit antibody|O GlcNAc transferase subunit p110 antibody|O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody|O linked N acetylglucosamine (GlcNAc) transferase antibody|O linked N acetylglucosamine transferase 110 kDa subunit antibody|O-GlcNAc transferase subunit p110 antibody|O-linked N-acetylglucosamine transferase 110 kDa subunit antibody|ogt antibody|OGT1_HUMAN antibody|UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody|UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody|UDP-N-acetylglucosamine–peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody|UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody|Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody|Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
SCBT cat No: sc-74547|sc-376253|sc-74546|sc-32921|sc-22628|
Rabbit polyclonal OGT antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human OGT
WB, IHC, IF, IP
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 117kDa/Observed: Refer to Figures
OGT antibody was tube-contained.
OGT antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
HRNT1 antibody, O-GLCNAC antibody
|Database links|| |
|Protein names|| |
|Protein function|| |
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the ‘Thr-308’ phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity . Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of ‘Ser-112’ of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) . As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues . O-GlcNAcylation of ‘Ser-75’ of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex . Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 .; Isoform 2: the mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.
|Protein tissue specificity|| |
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
|Protein sequence and domain|| |
The TPR repeat domain is required for substrate binding and oligomerization.Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.; Contains 13 TPR repeats.
|Protein post-translational modifications|| |
Ubiquitinated, leading to its proteasomal degradation.; Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity.
|Protein cellular localization|| |
Isoform 2: Mitochondrion. Membrane. Note: Associates with the mitochondrial inner membrane.; Isoform 3: Cytoplasm. Nucleus. Cell membrane. Note: Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane.; Isoform 4: Cytoplasm. Nucleus.
This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
AntibodyPlus can customize OGT antibody according to your requirement, including bulk product size,etc. Please contact email@example.com. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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