Rabbit Polyclonal p21 antibody (STJ94862)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal p21 antibody (STJ94862)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CDKN1A antibody, CAP20 antibody, CDKN1 antibody, CIP1 antibody, MDA6 antibody, PIC1 antibody, SDI1 antibody, WAF1 antibody,|CAP20 antibody|CDK-interacting protein 1 antibody|CDKI antibody|CDKN1 antibody|Cdkn1a antibody|CDN1A_HUMAN antibody|CIP1 antibody|Cyclin Dependent Kinase Inhibitor 1A antibody|Cyclin-dependent kinase inhibitor 1 antibody|Cyclin-dependent kinase inhibitor 1A (P21) antibody|Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody|DNA Synthesis Inhibitor antibody|MDA-6 antibody|MDA6 antibody|Melanoma differentiation-associated protein 6 antibody|Melanoma differentiation-associated protein antibody|p21 antibody|P21 protein antibody|p21CIP1 antibody|p21Cip1/Waf1 antibody|p21WAF antibody|PIC1 antibody|SDI1 antibody|SLC12A9 antibody|WAF1 antibody|Wild type p53 activated fragment 1 (WAF1) antibody|Wild type p53 activated fragment 1 antibody|Wildtype p53-activated fragment 1 antibody|Anti-p21 antibody [EPR362] (ab109520)
SCBT cat No: sc-71811|sc-817|sc-53393|sc-271532|sc-397|sc-56335|sc-6246|sc-271610|sc-756|sc-471|sc-469|sc-65595|sc-53870|sc-51689|sc-136020|sc-166630|sc-68396|sc-398612|sc-398750|

Name

p21 Polyclonal Antibody

Catalogue No.

STJ94862

Reactivity

Human, Mouse, Rat

Specificity

p21 Polyclonal Antibody detects endogenous levels of p21 protein.

Immunogen

Synthesized peptide derived from p21 at AA range 80-160

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

21 kDa

Formulation

p21 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

p21 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclin-dependent kinase inhibitor 1 antibody, CDK-interacting protein 1 antibody, Melanoma differentiation-associated protein 6 antibody, MDA-6 antibody, p21 antibody

Database links

Human UniProt/Swiss-Prot:P38936;Mouse UniPort/Swiss-Prot: P39689;Rat UniProt/Swiss-Port: Q64315;Human Entrez Gene: 1026;Mouse Entrez Gene: 12575;Rat Entrez Gene: Rn.10089

Protein names

Cyclin-dependent kinase inhibitor 1 , CDK-interacting protein 1 , Melanoma differentiation-associated protein 6 , MDA-6 , p21

Protein function

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Protein tissue specificity

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Protein sequence and domain

The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination. / The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. / Belongs to the CDI family.

Protein post-translational modifications

Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. / Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the ‘K+4’ motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. / Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Cell Cycle Proteins>p21
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize p21 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunofluorescence analysis of Mouse kidney tissue

1: p21 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunofluorescence analysis of Rat lung tissue

1: p21 Polyclonal Antibody(red) was diluted at 1:200 (4 degree Celsius,overnight).
2: Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).
3: Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: p21 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: p21 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse lung tissue

1: p21 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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