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Rabbit Polyclonal p21 antibody (STJ97470)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC
Recommended dilution: WB 1:1000-2000; IHC 1:100-200
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
CDKN1A antibody, CAP20 antibody, CDKN1 antibody, CIP1 antibody, MDA6 antibody, PIC1 antibody, SDI1 antibody, WAF1 antibody,|CAP20 antibody|CDK-interacting protein 1 antibody|CDKI antibody|CDKN1 antibody|Cdkn1a antibody|CDN1A_HUMAN antibody|CIP1 antibody|Cyclin Dependent Kinase Inhibitor 1A antibody|Cyclin-dependent kinase inhibitor 1 antibody|Cyclin-dependent kinase inhibitor 1A (P21) antibody|Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody|DNA Synthesis Inhibitor antibody|MDA-6 antibody|MDA6 antibody|Melanoma differentiation-associated protein 6 antibody|Melanoma differentiation-associated protein antibody|p21 antibody|P21 protein antibody|p21CIP1 antibody|p21Cip1/Waf1 antibody|p21WAF antibody|PIC1 antibody|SDI1 antibody|SLC12A9 antibody|WAF1 antibody|Wild type p53 activated fragment 1 (WAF1) antibody|Wild type p53 activated fragment 1 antibody|Wildtype p53-activated fragment 1 antibody|Anti-p21 antibody [EPR362] (ab109520)
SCBT cat No: sc-71811|sc-817|sc-53393|sc-271532|sc-397|sc-56335|sc-6246|sc-271610|sc-756|sc-471|sc-469|sc-65595|sc-53870|sc-51689|sc-136020|sc-166630|sc-68396|sc-398612|sc-398750|
p21 Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
The antibody detects endogenous p21 protein.
Synthesized peptide derived from human p21 around the non-phosphorylation site of T145
|Recommended dilution|| |
WB 1:1000-2000; IHC 1:100-200
p21 Antibody was tube-contained. PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
p21 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Cyclin-dependent kinase inhibitor 1 antibody, CDK-interacting protein 1 antibody, Melanoma differentiation-associated protein 6 antibody, MDA-6 antibody, p21 antibody
|Protein names|| |
Cyclin-dependent kinase inhibitor 1 , CDK-interacting protein 1 , Melanoma differentiation-associated protein 6 , MDA-6 , p21
|Protein function|| |
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
|Protein tissue specificity|| |
Expressed in all adult tissues, with 5-fold lower levels observed in the brain.
|Protein sequence and domain|| |
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination. / The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. / Belongs to the CDI family.
|Protein post-translational modifications|| |
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. / Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the ‘K+4’ motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation. / Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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