Rabbit Polyclonal p27 antibody (STJ94866)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal p27 antibody (STJ94866)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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CDKN1B antibody, KIP1 antibody,|AA408329 antibody|AI843786 antibody|Cdki1b antibody|CDKN 1B antibody|CDKN 4 antibody|CDKN1B antibody|CDKN4 antibody|CDN1B_HUMAN antibody|Cyclin Dependent Kinase Inhibitor 1B antibody|Cyclin dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|Cyclin-dependent kinase inhibitor 1B antibody|Cyclin-dependent kinase inhibitor p27 antibody|Cyclin-dependent kinase inhibitor p27 Kip1 antibody|KIP 1 antibody|KIP1 antibody|MEN1B antibody|MEN4 antibody|OTTHUMP00000195098 antibody|OTTHUMP00000195099 antibody|p27 antibody|p27 Kip1 antibody|P27-like cyclin-dependent kinase inhibitor antibody|p27Kip1 antibody|Anti-p27 KIP 1 antibody [Y236] (ab32034)
SCBT cat No: sc-393380|sc-528|sc-393305|sc-56338|sc-1641|sc-776|sc-527|sc-56454|sc-53906|sc-53871|sc-6359|sc-9962|

Name

p27 Polyclonal Antibody

Catalogue No.

STJ94866

Reactivity

Human

Specificity

p27 Polyclonal Antibody detects endogenous levels of p27 protein.

Immunogen

Synthesized peptide derived from p27 at AA range 140-220

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

27 kDa

Formulation

p27 Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

p27 Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclin-dependent kinase inhibitor 1B antibody, Cyclin-dependent kinase inhibitor p27 antibody, p27Kip1 antibody

Database links

Human UniProt/Swiss-Prot:P46527;Mouse UniPort/Swiss-Prot: P46414;Rat UniProt/Swiss-Port: O08769;Human Entrez Gene: 1027;Mouse Entrez Gene: 12576;Rat Entrez Gene: Rn.29897

Protein names

Cyclin-dependent kinase inhibitor 1B , Cyclin-dependent kinase inhibitor p27 , p27Kip1

Protein function

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Protein tissue specificity

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Involvement in disease

Multiple endocrine neoplasia 4 (MEN4) [MIM:610755]: Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity. / Belongs to the CDI family.

Protein post-translational modifications

Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G1 phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF. / Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation. / Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).

Protein cellular localization

Nucleus / Cytoplasm / Endosome

Research area

All research areas>Cell Cycle Proteins>p27
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize p27 Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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Immunohistochemical analysis of paraffin embedded Human liver cancer tissue

1: p27 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue

1: p27 Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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