Rabbit polyclonal P4HB antibody (A0692)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF,IP,RIP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal P4HB antibody (A0692)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF,IP,RIP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:20 – 1:100 IP 1:20 – 1:50 RIP 1:20 – 1:50

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

P4HB antibody,DSI antibody,ERBA2L antibody,GIT antibody,P4Hbeta antibody,PDI antibody,PDIA1 antibody,PHDB antibody,PO4DB antibody,PO4HB antibody,PROHB antibody
P4HB antibody|Pancreas specific protein disulfide isomerase antibody|Pancreas-specific protein disulfide isomerase antibody|Pancreatic protein disulfide isomerase antibody|PDA2 antibody|PDI antibody|PDIA2 antibody|PDIA2_HUMAN antibody|PDIp antibody|PDIR antibody|Protein disulfide isomerase A2 antibody|Protein disulfide isomerase antibody|Protein disulfide isomerase associated 2 antibody|Protein disulfide isomerase family A member 2 antibody|Protein disulfide isomerase pancreatic antibody|Protein disulfide-isomerase A2 antibody|Rho GDP dissociation inhibitor gamma antibody|Anti-PDI antibody [RL90] (ab2792)

SCBT cat No: sc-136230|sc-376370|sc-74551|sc-376369|sc-17222|sc-166474|sc-30931|sc-20132|sc-30932|

Name

Rabbit polyclonal P4HB antibody

Catalogue No.

A0692

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human P4HB

Host

Rabbit

Applications

WB, IHC, IF, IP, RIP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:20 – 1:100
IP 1:20 – 1:50
RIP 1:20 – 1:50

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 57kDa/Observed: Refer to Figures

Formulation

P4HB antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

P4HB antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

P4HB antibody,DSI antibody,ERBA2L antibody,GIT antibody,P4Hbeta antibody,PDI antibody,PDIA1 antibody,PHDB antibody,PO4DB antibody,PO4HB antibody,PROHB antibody

Database links

Human UniProt/Swiss-Prot:P07237

Protein names

P4HB,DSI,ERBA2L,GIT,P4Hbeta,PDI,PDIA1,PHDB,PO4DB,PO4HB,PROHB

Protein function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration .

Protein sequence and domain

Belongs to the protein disulfide isomerase family.; Contains 2 thioredoxin domains.

Protein cellular localization

Endoplasmic reticulum . Endoplasmic reticulum lumen . Melanosome . Cell membrane ; Peripheral membrane protein . Note: Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces . Identified by mass spectrometry in melanosome fractions from stage I to stage IV . Colocalizes with MTTP in the endoplasmic reticulum .

Background

During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).

Note

AntibodyPlus can customize P4HB antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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