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Rabbit polyclonal PAK1 antibody (A2554)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,ICC,IP
WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:50 – 1:200 IP 1:20 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal PAK1 antibody.
Check alternative names for the antibodyExpand
MGC130000 antibody, MGC130001 antibody, PAKalpha antibody
ADRB2 antibody|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody|p65 PAK antibody|p65-PAK antibody|P68-PAK antibody|PAK alpha antibody|PAK-1 antibody|Pak1 antibody|PAK1_HUMAN antibody|Paka antibody|PAKalpha antibody|Protein kinase MUK2 antibody|Rac/p21-activated kinase antibody|Serine/threonine-protein kinase PAK 1 antibody|STE20 homolog yeast antibody|Anti-PAK1 antibody [EP795Y] (ab40852)
SCBT cat No: sc-882|sc-50693|sc-50694|sc-293362|sc-10925|sc-10927|sc-161294|
Rabbit polyclonal PAK1 antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human PAK1
WB, IHC, ICC, IP
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 62kDa/Observed: Refer to Figures
PAK1 antibody was tube-contained.
PAK1 antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
MGC130000 antibody, MGC130001 antibody, PAKalpha antibody
|Database links|| |
|Protein names|| |
MGC130000, MGC130001, PAKalpha
|Protein function|| |
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at ‘Ser-338’ and ‘Ser-339’ resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at ‘Ser-246’ promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion .
|Protein tissue specificity|| |
Overexpressed in gastric cancer cells and tissues (at protein level) .
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.; Contains 1 CRIB domain.; Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites.
|Protein cellular localization|| |
Cytoplasm . Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Cell projection, invadopodium . Note: Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery . Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin.
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1 and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10).
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|Product type|| |
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