Rabbit polyclonal PAK4 antibody (A2782)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PAK4 antibody (A2782)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000 IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PAK4 antibody.

Alternative names:

Check alternative names for the antibody

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KIAA1142 antibody|Serine threonine kinase PAK 4 antibody|Serine/threonine protein kinase PAK 4 antibody|Serine/threonine protein kinase PAK4 antibody|Serine/threonine-protein kinase PAK 4 antibody|Anti-PAK4 antibody (ab62509)

SCBT cat No: sc-81532|sc-390507|sc-393367|sc-28779|sc-18622|sc-22155|sc-393102|

Name

Rabbit polyclonal PAK4 antibody

Catalogue No.

A2782

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PAK4

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 64kD/Observed: Refer to Figures

Formulation

PAK4 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PAK4 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Database links

Human UniProt/Swiss-Prot:O96013

Protein function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

Protein tissue specificity

Highest expression in prostate, testis and colon.

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.; Contains 1 CRIB domain.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated on serine residues when activated by CDC42/p21.; Phosphorylated on tyrosine residues upon stimulation of FGFR2.

Protein cellular localization

Cytoplasm . Note: Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways.

Background

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Research area

All research areas>Kinases and Phosphatases>PAK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize PAK4 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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