Rabbit polyclonal PARK2 antibody (A0968)

$69.00$259.00

Reactivity: Mouse,Rat
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PARK2 antibody (A0968)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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PARK2 antibody,AR-JP antibody,LPRS2 antibody,PDJ antibody,PRKN antibody,Parkin antibody
AR JP antibody|E3 ubiquitin ligase antibody|E3 ubiquitin protein ligase parkin antibody|E3 ubiquitin-protein ligase parkin antibody|FRA6E antibody|LPRS 2 antibody|LPRS2 antibody|PARK 2 antibody|Park2 antibody|Parkin 2 antibody|Parkinson disease (autosomal recessive juvenile) 2 antibody|Parkinson disease (autosomal recessive, juvenile) 2, parkin antibody|Parkinson disease protein 2 antibody|Parkinson juvenile disease protein 2 antibody|Parkinson protein 2 E3 ubiquitin protein ligase antibody|Parkinson protein 2, E3 ubiquitin protein ligase (parkin) antibody|PDJ antibody|PRKN 2 antibody|PRKN antibody|PRKN2 antibody|PRKN2_HUMAN antibody|Ubiquitin E3 ligase PRKN antibody|Anti-Parkin antibody [PRK8] (ab77924)

SCBT cat No: sc-133167|sc-30130|sc-136989|sc-13279|sc-32282|sc-31603|sc-133884|

Name

Rabbit polyclonal PARK2 antibody

Catalogue No.

A0968

Reactivity

Mouse, Rat

Immunogen

Recombinant protein of human PARK2

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 52kDa/Observed: Refer to Figures

Formulation

PARK2 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PARK2 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PARK2 antibody,AR-JP antibody,LPRS2 antibody,PDJ antibody,PRKN antibody,Parkin antibody

Database links

Human UniProt/Swiss-Prot:O60260

Protein names

PARK2,AR-JP,LPRS2,PDJ,PRKN,Parkin

Protein function

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2 . Mediates monoubiquitination as well as ‘Lys-6’, ‘Lys-11’, ‘Lys-48’-linked and ‘Lys-63’-linked polyubiquitination of substrates depending on the context . Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating ‘Lys-63’-linked polyubiquitination of misfolded proteins such as PARK7: ‘Lys-63’-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation . Mediates ‘Lys-63’-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation . Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy . Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1 and USP30 . Preferentially assembles ‘Lys-6’-, ‘Lys-11’- and ‘Lys-63’-linked polyubiquitin chains following mitochondrial damage, leading to mitophagy . Mediates ‘Lys-48’-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death . Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress . Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 . May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity . May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene.

Protein tissue specificity

Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level).

Protein sequence and domain

The ubiquitin-like domain binds the PSMD4 subunit of 26S proteasomes.; The RING-type 1 zinc finger domain is required to repress p53/TP53 transcription.Belongs to the RBR family. Parkin subfamily.; Contains 1 IBR-type zinc finger.; Contains 3 RING-type zinc fingers.; Contains 1 ubiquitin-like domain.

Protein post-translational modifications

Auto-ubiquitinates in an E2-dependent manner leading to its own degradation . Also polyubiquitinated by RNF41 for proteasomal degradation.; S-nitrosylated. The inhibition of PARK2 ubiquitin E3 ligase activity by S-nitrosylation could contribute to the degenerative process in PD by impairing the ubiquitination of PARK2 substrates.; Phosphorylation at Ser-65 by PINK1 contributes to activate PARK2 activity. It is however not sufficient and requires binding to phosphorylated ubiquitin as well.

Protein cellular localization

Cytoplasm, cytosol . Nucleus. Endoplasmic reticulum. Mitochondrion . Note: Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Mitochondrial localization gradually increases with cellular growth. Also relocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent.

Background

Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2).

Research area

All research areas>Neurobiology>Parkin
(View all antibody categories related to Neurobiology)

Note

AntibodyPlus can customize PARK2 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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