Rabbit polyclonal PARK7 antibody (A0987)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,ICC,IF,IP
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PARK7 antibody (A0987)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,ICC,IF,IP

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 ICC 1:20 – 1:100 IF 1:50 – 1:200 IP 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PARK7 antibody.

Alternative names:

Check alternative names for the antibody

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PARK7 antibody,DJ-1 antibody,DJ1 antibody,FLJ27376 antibody,FLJ34360 antibody,FLJ92274 antibody
CAP1 antibody|DJ-1 antibody|DJ1 antibody|DJ1 protein antibody|Epididymis secretory sperm binding protein Li 67p antibody|FLJ27376 antibody|FLJ34360 antibody|FLJ92274 antibody|HEL S 67p antibody|Oncogene DJ1 antibody|OTTHUMP00000001348 antibody|OTTHUMP00000001349 antibody|OTTHUMP00000001350 antibody|OTTHUMP00000001351 antibody|PARK7 antibody|PARK7_HUMAN antibody|Parkinson disease (autosomal recessive, early onset) 7 antibody|Parkinson disease protein 7 antibody|Parkinson protein 7 antibody|Protein DJ-1 antibody|SP22 antibody|Anti-PARK7/DJ1 antibody (ab18257)

SCBT cat No: sc-55573|sc-27006|sc-55572|sc-32874|sc-33665|sc-27004|

Name

Rabbit polyclonal PARK7 antibody

Catalogue No.

A0987

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PARK7

Host

Rabbit

Applications

WB, IHC, ICC, IF, IP

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
ICC 1:20 – 1:100
IF 1:50 – 1:200
IP 1:20 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 20kDa/Observed: Refer to Figures

Formulation

PARK7 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PARK7 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PARK7 antibody,DJ-1 antibody,DJ1 antibody,FLJ27376 antibody,FLJ34360 antibody,FLJ92274 antibody

Database links

Human UniProt/Swiss-Prot:Q99497

Protein names

PARK7,DJ-1,DJ1,FLJ27376,FLJ34360,FLJ92274

Protein function

Protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteines, arginines and lysines residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) . Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function . It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway . Its involvement in protein repair could also explain other unrelated functions. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death . Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria . Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking . Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells . Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress . Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity .

Protein tissue specificity

Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

Protein sequence and domain

Belongs to the peptidase C56 family.

Protein post-translational modifications

Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.; Cys-106 is easily oxidized to sulfinic acid.; Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.

Protein cellular localization

Cell membrane ; Lipid-anchor . Cytoplasm . Nucleus . Membrane raft . Mitochondrion . Note: Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage . Detected in tau inclusions in brains from neurodegenerative disease patients . Membrane raft localization in astrocytes and neuronal cells requires palmitoylation.

Background

Parkinson’s disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in alpha-synuclein, Parkin, and DJ-1 are linked to PD (1). alpha-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

Research area

All research areas>Tumor Suppressor/Apoptosis>DJ-1
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize PARK7 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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