Rabbit Polyclonal PCNA antibody (STJ94982)

$99.00$319.00

Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal PCNA antibody (STJ94982)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

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Alternative names:

Check alternative names for the antibody

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PCNA antibody,|ATLD2 antibody|cb16 antibody|Cyclin antibody|DNA polymerase delta auxiliary protein antibody|etID36690.10 antibody|fa28e03 antibody|fb36g03 antibody|HGCN8729 antibody|MGC8367 antibody|Mutagen-sensitive 209 protein antibody|OTTHUMP00000030189 antibody|OTTHUMP00000030190 antibody|PCNA antibody|Pcna/cyclin antibody|PCNA_HUMAN antibody|PCNAR antibody|Polymerase delta accessory protein antibody|Proliferating cell nuclear antigen antibody|wu:fa28e03 antibody|wu:fb36g03 antibody|Anti-PCNA antibody (ab18197)
SCBT cat No: sc-71858|sc-9857|sc-25280|sc-7907|sc-56|sc-53407|sc-53408|sc-65598|

 

Name

PCNA Polyclonal Antibody

Catalogue No.

STJ94982

Reactivity

Human, Mouse, Rat, Monkey

Specificity

PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.

Immunogen

Synthesized peptide derived from PCNA at AA range 30-110

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

36 kDa

Formulation

PCNA Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

PCNA Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Proliferating cell nuclear antigen antibody, PCNA antibody, Cyclin antibody

Database links

Human UniProt/Swiss-Prot:P12004;Mouse UniPort/Swiss-Prot: P17918;Rat UniProt/Swiss-Port: P04961;Human Entrez Gene: 5111;Mouse Entrez Gene: 18538;Rat Entrez Gene: Rn.223

Protein names

Proliferating cell nuclear antigen , PCNA , Cyclin

Protein function

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while ‘Lys-63’-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Involvement in disease

Ataxia-telangiectasia-like disorder 2 (ATLD2) [MIM:615919]: A neurodegenerative disorder due to defects in DNA excision repair. ATLD2 is characterized by developmental delay, ataxia, sensorineural hearing loss, short stature, cutaneous and ocular telangiectasia, and photosensitivity. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the PCNA family.

Protein post-translational modifications

Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. / Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation . Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner . Acetylation disrupts interaction with NUDT15 and promotes degradation . / Ubiquitinated . Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through ‘Lys-63’ linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. / Methylated on glutamate residues by ARMT1/C6orf211.

Protein cellular localization

Nucleus

Research area

All research areas>Cell Cycle Proteins>PCNA
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize PCNA Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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