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Rabbit Polyclonal PCNA antibody (STJ94982)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
Recommended protocols: check protocols
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Check alternative names for the antibodyExpand
PCNA antibody,|ATLD2 antibody|cb16 antibody|Cyclin antibody|DNA polymerase delta auxiliary protein antibody|etID36690.10 antibody|fa28e03 antibody|fb36g03 antibody|HGCN8729 antibody|MGC8367 antibody|Mutagen-sensitive 209 protein antibody|OTTHUMP00000030189 antibody|OTTHUMP00000030190 antibody|PCNA antibody|Pcna/cyclin antibody|PCNA_HUMAN antibody|PCNAR antibody|Polymerase delta accessory protein antibody|Proliferating cell nuclear antigen antibody|wu:fa28e03 antibody|wu:fb36g03 antibody|Anti-PCNA antibody (ab18197)
SCBT cat No: sc-71858|sc-9857|sc-25280|sc-7907|sc-56|sc-53407|sc-53408|sc-65598|
PCNA Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.
Synthesized peptide derived from PCNA at AA range 30-110
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
|Molecular weight|| |
PCNA Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PCNA Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Proliferating cell nuclear antigen antibody, PCNA antibody, Cyclin antibody
|Protein names|| |
Proliferating cell nuclear antigen , PCNA , Cyclin
|Protein function|| |
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase’s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3′-5′ exonuclease and 3′-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways . Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while ‘Lys-63’-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
|Involvement in disease|| |
Ataxia-telangiectasia-like disorder 2 (ATLD2) [MIM:615919]: A neurodegenerative disorder due to defects in DNA excision repair. ATLD2 is characterized by developmental delay, ataxia, sensorineural hearing loss, short stature, cutaneous and ocular telangiectasia, and photosensitivity. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the PCNA family.
|Protein post-translational modifications|| |
Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA. / Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation . Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner . Acetylation disrupts interaction with NUDT15 and promotes degradation . / Ubiquitinated . Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through ‘Lys-63’ linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase. / Methylated on glutamate residues by ARMT1/C6orf211.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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