No products in the cart.
Rabbit polyclonal PDGFRA antibody (A2103)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000 IHC 1:50 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit polyclonal PDGFRA antibody.
Check alternative names for the antibodyExpand
CD140A antibody, MGC74795 antibody, PDGFR2 antibody, Rhe-PDGFRA antibody
Alpha-type platelet-derived growth factor receptor antibody|CD140 antigen-like family member A antibody|CD140a antibody|CD140a antigen antibody|MGC74795 antibody|PDGF alpha chain antibody|PDGF-R-alpha antibody|PDGFR 2 antibody|PDGFR alpha antibody|PDGFR2 antibody|PDGFRA antibody|PDGFRA/BCR fusion antibody|PGFRA_HUMAN antibody|Platelet derived growth factor receptor 2 antibody|Platelet derived growth factor receptor alpha antibody|Platelet derived growth factor receptor alpha polypeptide antibody|Platelet derived growth factor receptor antibody|Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody|RHEPDGFRA antibody|Phospho anti-PDGF Receptor alpha (Y754) antibody (ab5460)
SCBT cat No: sc-101569|
Rabbit polyclonal PDGFRA antibody
|Catalogue No.|| |
Human, Mouse, Rat
Recombinant protein of human PDGFRA
|Recommended dilution|| |
WB 1:500 – 1:2000
|Molecular weight|| |
Predicted: 123kDa/Observed: Refer to Figures
PDGFRA antibody was tube-contained.
PDGFRA antibody was purified using affinity purification.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
CD140A antibody, MGC74795 antibody, PDGFR2 antibody, Rhe-PDGFRA antibody
|Database links|| |
|Protein names|| |
CD140A, MGC74795, PDGFR2, Rhe-PDGFRA
|Protein function|| |
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands – homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
|Protein tissue specificity|| |
Detected in platelets (at protein level). Widely expressed. Detected in brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.; Contains 5 Ig-like C2-type (immunoglobulin-like) domains.; Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
N-glycosylated.; Ubiquitinated, leading to its degradation.; Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-731 and Tyr-742 is important for interaction with PIK3R1. Phosphorylation at Tyr-720 and Tyr-754 is important for interaction with PTPN11. Phosphorylation at Tyr-762 is important for interaction with CRK. Phosphorylation at Tyr-572 and Tyr-574 is important for interaction with SRC and SRC family members. Phosphorylation at Tyr-988 and Tyr-1018 is important for interaction with PLCG1.
|Protein cellular localization|| |
Cell membrane ; Single-pass type I membrane protein . Note: The activated receptor is rapidly internalized and degraded.
Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor alpha (PDGFRalpha) and PDGF receptor beta (PDGFRbeta). PDGFRalpha and PDGFRbeta share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRalpha homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRbeta homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor alpha/beta binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRalpha and PDGFRbeta can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCgamma, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRbeta is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRbeta (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRbeta (7). Tyr740 is also required for PDGFRbeta-mediated PI3 kinase activation (8).
AntibodyPlus can customize PDGFRA antibody according to your requirement, including bulk product size,etc. Please contact email@example.com. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
There are no reviews for this product yet.
By submitting a review, get following benefits:
1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!
There is no extra validation for this product yet.
Check other extra validated antibodies below: