Rabbit polyclonal PDHA1 antibody (A1895)

$69.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PDHA1 antibody (A1895)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal PDHA1 antibody.

Alternative names:

Check alternative names for the antibody

Expand

PDHA antibody, PDHCE1A antibody, PHE1A antibody
ODPA_HUMAN antibody|PDH antibody|PDHA antibody|PDHA1 antibody|PDHCE1A antibody|PDHE1 A type I antibody|PDHE1-A type I antibody|PHE1A antibody|Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody|Pyruvate dehydrogenase complex, E1 alpha polypeptide 1 antibody|Pyruvate Dehydrogenase E1 alpha antibody|Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial antibody|Phospho anti-Pyruvate Dehydrogenase E1-alpha subunit (S293) antibody (ab92696)

SCBT cat No: sc-377092|sc-133898|sc-292543|

Name

Rabbit polyclonal PDHA1 antibody

Catalogue No.

A1895

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PDHA1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 43kDa/Observed: Refer to Figures

Formulation

PDHA1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PDHA1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PDHA antibody, PDHCE1A antibody, PHE1A antibody

Database links

Human UniProt/Swiss-Prot:P08559

Protein names

PDHA, PDHCE1A, PHE1A

Protein function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Protein tissue specificity

Ubiquitous.

Protein post-translational modifications

Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.; Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

Protein cellular localization

Mitochondrion matrix.

Background

The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: alpha and beta. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the alpha subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2).

Research area

All research areas>Signaling Intermediates>PDH-E1
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize PDHA1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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