Rabbit polyclonal PECAM1 antibody (A3181)

$69.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal PECAM1 antibody (A3181)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:25 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CD31 antibody, PECA1 antibody, GPIIA’ antibody, PECAM-1 antibody, endoCAM antibody
Adhesion molecule antibody|CD31 antibody|CD31 antigen antibody|CD31 EndoCAM antibody|EndoCAM antibody|FLJ34100 antibody|FLJ58394 antibody|GPIIA antibody|GPIIA’ antibody|PECA1 antibody|PECA1_HUMAN antibody|Pecam 1 antibody|PECAM 1 CD31 EndoCAM antibody|PECAM antibody|PECAM-1 antibody|Pecam1 antibody|Platelet and endothelial cell adhesion molecule 1 antibody|Platelet endothelial cell adhesion molecule antibody|Platelet/endothelial cell adhesion molecule 1 antibody|Anti-CD31 antibody (ab28364)

SCBT cat No: sc-71871|sc-1505|sc-46694|sc-365804|sc-133091|

Name

Rabbit polyclonal PECAM1 antibody

Catalogue No.

A3181

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human PECAM1

Host

Rabbit

Applications

WB, IHC

Recommended dilution

WB 1:500 – 1:2000
IHC 1:25 – 1:100

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 83kDa/Observed: Refer to Figures

Formulation

PECAM1 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

PECAM1 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CD31 antibody, PECA1 antibody, GPIIA’ antibody, PECAM-1 antibody, endoCAM antibody

Database links

Human UniProt/Swiss-Prot:P16284

Protein names

CD31, PECA1, GPIIA’, PECAM-1, endoCAM

Protein function

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting ‘detachment’ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell’s active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Protein tissue specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).

Protein sequence and domain

The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Protein post-translational modifications

Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.; Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.

Protein cellular localization

Isoform Long: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.; Isoform Delta15: Cell junction . Note: Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

Research area

All research areas>Cell Adhesion Proteins>PECAM
(View all antibody categories related to Cell Adhesion Proteins)

Note

AntibodyPlus can customize PECAM1 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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