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Rabbit Polyclonal Phospho-14-3-3 beta/zeta (S184/186) antibody (STJ90562)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for 14-3-3 beta/zeta (phospho Ser184/186) Polyclonal Antibody.
Check alternative names for the antibodyExpand
YWHAZ antibody,|14 3 3 delta antibody|14 3 3 protein zeta/delta antibody|14 3 3 protein/cytosolic phospholipase A2 antibody|14 3 3 zeta antibody|14-3-3 protein zeta/delta antibody|1433Z_HUMAN antibody|Epididymis luminal protein 4 antibody|Epididymis secretory protein Li 3 antibody|HEL S 3 antibody|HEL4 antibody|KCIP-1 antibody|KCIP1 antibody|MGC111427 antibody|MGC126532 antibody|MGC138156 antibody|Phospholipase A2 antibody|Protein kinase C inhibitor protein 1 antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|YWHAD antibody|YWHAZ antibody|Anti-14-3-3 zeta antibody (ab51129)
SCBT cat No: sc-293415|sc-1019|sc-31964|sc-31965|
14-3-3 beta/zeta (phospho Ser184/186) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-14-3-3 beta/zeta (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 beta/zeta protein only when phosphorylated at S184/186.
Synthesized phospho-peptide derived from 14-3-3 beta/zeta (phospho Ser184/186) at AA range 120-200
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:5000;
|Molecular weight|| |
14-3-3 beta/zeta (phospho Ser184/186) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
14-3-3 beta/zeta (phospho Ser184/186) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
14-3-3 protein zeta/delta antibody, Protein kinase C inhibitor protein 1 antibody, KCIP-1 antibody
|Protein names|| |
14-3-3 protein zeta/delta , Protein kinase C inhibitor protein 1 , KCIP-1
|Protein function|| |
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
|Protein sequence and domain|| |
Belongs to the 14-3-3 family.
|Protein post-translational modifications|| |
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.
|Protein cellular localization|| |
Cytoplasm / Melanosome
AntibodyPlus can customize 14-3-3 beta/zeta (phospho Ser184/186) Antibody according to your requirement, including bulk product size,etc. Please contact email@example.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.
St John’s Laboratory Ltd.
|Product type|| |
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Antibody customer reviews & validation data - 39
Validation data ID: 39
Submission date: 2016-12-21
Five cell line lysates normalysed to equal concentration and loaded in duplicates. The presence of the bands in the expected region and absence of any other bands point toward specificity of the antibodies. I would like to try these antibodies again with milk as a blocking buffer to reduce background.
Rabbit Polyclonal Phospho-14-3-3 beta/zeta (S184/186) antibody
Product catalog No.
St John's Laboratory Ltd
Antibody specificity rating
Antibody overall rating
I have tried these antibodies only for WB and received bands in expected region for all five cell lines. My blot was a little too messy due to the use of BSA as a blocking buffer.
There is no extra validation for this product yet.
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