Rabbit Polyclonal Phospho-14-3-3 beta/zeta (S184/186) antibody (STJ90562)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-14-3-3 beta/zeta (S184/186) antibody (STJ90562)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for 14-3-3 beta/zeta (phospho Ser184/186) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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YWHAZ antibody,|14 3 3 delta antibody|14 3 3 protein zeta/delta antibody|14 3 3 protein/cytosolic phospholipase A2 antibody|14 3 3 zeta antibody|14-3-3 protein zeta/delta antibody|1433Z_HUMAN antibody|Epididymis luminal protein 4 antibody|Epididymis secretory protein Li 3 antibody|HEL S 3 antibody|HEL4 antibody|KCIP-1 antibody|KCIP1 antibody|MGC111427 antibody|MGC126532 antibody|MGC138156 antibody|Phospholipase A2 antibody|Protein kinase C inhibitor protein 1 antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|YWHAD antibody|YWHAZ antibody|Anti-14-3-3 zeta antibody (ab51129)
SCBT cat No: sc-293415|sc-1019|sc-31964|sc-31965|

 

Name

14-3-3 beta/zeta (phospho Ser184/186) Polyclonal Antibody

Catalogue No.

STJ90562

Reactivity

Human, Mouse, Rat

Specificity

Phospho-14-3-3 beta/zeta (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 beta/zeta protein only when phosphorylated at S184/186.

Immunogen

Synthesized phospho-peptide derived from 14-3-3 beta/zeta (phospho Ser184/186) at AA range 120-200

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

28 kDa

Formulation

14-3-3 beta/zeta (phospho Ser184/186) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

14-3-3 beta/zeta (phospho Ser184/186) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

14-3-3 protein zeta/delta antibody, Protein kinase C inhibitor protein 1 antibody, KCIP-1 antibody

Database links

Human UniProt/Swiss-Prot:P63104 ;Human Entrez Gene: 7534;

Protein names

14-3-3 protein zeta/delta , Protein kinase C inhibitor protein 1 , KCIP-1

Protein function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Protein sequence and domain

Belongs to the 14-3-3 family.

Protein post-translational modifications

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Protein cellular localization

Cytoplasm / Melanosome

Note

AntibodyPlus can customize 14-3-3 beta/zeta (phospho Ser184/186) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 39

Validation data ID: 39

Submission date: 2016-12-21

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Result figure:
Result figure

Figure description:

Five cell line lysates normalysed to equal concentration and loaded in duplicates. The presence of the bands in the expected region and absence of any other bands point toward specificity of the antibodies. I would like to try these antibodies again with milk as a blocking buffer to reduce background.

Product name

Rabbit Polyclonal Phospho-14-3-3 beta/zeta (S184/186) antibody

Product catalog No.

STJ90562

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

I have tried these antibodies only for WB and received bands in expected region for all five cell lines. My blot was a little too messy due to the use of BSA as a blocking buffer.

Detailed protocol

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