Rabbit Polyclonal Phospho-14-3-3 zeta/delta (T232) antibody (STJ90869)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-14-3-3 zeta/delta (T232) antibody (STJ90869)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for 14-3-3 zeta/delta (phospho Thr232) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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YWHAZ antibody,|14 3 3 delta antibody|14 3 3 protein zeta/delta antibody|14 3 3 protein/cytosolic phospholipase A2 antibody|14 3 3 zeta antibody|14-3-3 protein zeta/delta antibody|1433Z_HUMAN antibody|Epididymis luminal protein 4 antibody|Epididymis secretory protein Li 3 antibody|HEL S 3 antibody|HEL4 antibody|KCIP-1 antibody|KCIP1 antibody|MGC111427 antibody|MGC126532 antibody|MGC138156 antibody|Phospholipase A2 antibody|Protein kinase C inhibitor protein 1 antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta antibody|Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|Tyrosine 3/tryptophan 5 monooxygenase activation protein, zeta polypeptide antibody|YWHAD antibody|YWHAZ antibody|Anti-14-3-3 zeta antibody (ab51129)
SCBT cat No: sc-293415|sc-1019|sc-31964|sc-31965|

 

Name

14-3-3 zeta/delta (phospho Thr232) Polyclonal Antibody

Catalogue No.

STJ90869

Reactivity

Human, Mouse, Rat

Specificity

Phospho-14-3-3 zeta/delta (T232) Polyclonal Antibody detects endogenous levels of 14-3-3 zeta/delta protein only when phosphorylated at T232.

Immunogen

Synthesized phospho-peptide derived from 14-3-3 zeta/delta (phospho Thr232) at AA range 170-250

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

28 kDa

Formulation

14-3-3 zeta/delta (phospho Thr232) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

14-3-3 zeta/delta (phospho Thr232) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

14-3-3 protein zeta/delta antibody, Protein kinase C inhibitor protein 1 antibody, KCIP-1 antibody

Database links

Human UniProt/Swiss-Prot:P63104;Mouse UniPort/Swiss-Prot: P63101;Rat UniProt/Swiss-Port: P63102;Human Entrez Gene: 7534;Mouse Entrez Gene: 22631;Rat Entrez Gene: Rn.1292

Protein names

14-3-3 protein zeta/delta , Protein kinase C inhibitor protein 1 , KCIP-1

Protein function

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

Protein sequence and domain

Belongs to the 14-3-3 family.

Protein post-translational modifications

The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53.

Protein cellular localization

Cytoplasm / Melanosome

Note

AntibodyPlus can customize 14-3-3 zeta/delta (phospho Thr232) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 33

Validation data ID: 33

Submission date: 2016-12-07

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Result figure:
Result figure

Figure description:

Five human cell lysates loaded in pairs into 10% gel. All five cell lines show presence of phosphorylated 14-3-3.
The 5% milk in TBST was used as a blocking buffer; however, it seem not to negatively affect the results.

Product name

Rabbit Polyclonal Phospho-14-3-3 zeta/delta (T232) antibody

Product catalog No.

STJ90869

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Western Blot

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

I was able to detect the bands in all five of my cell lines.

Detailed protocol

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