Rabbit Polyclonal Phospho-4E-BP1 (T46) antibody (STJ90162)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-4E-BP1 (T46) antibody (STJ90162)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for 4E-BP1 (phospho Thr46) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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EIF4EBP1 antibody,|4E-BP1 antibody|4EBP1 antibody|4EBP1_HUMAN antibody|BP 1 antibody|eIF4E binding protein 1 antibody|eIF4E-binding protein 1 antibody|Eif4ebp1 antibody|Eukaryotic translation initiation factor 4E-binding protein 1 antibody|PHAS-I antibody|PHASI antibody|Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody|Anti-eIF4EBP1 antibody (ab2606)
SCBT cat No: sc-81149|sc-6024|sc-514073|sc-6025|sc-9977|sc-6936|sc-74397|sc-134232|sc-81175|sc-393788|sc-13454|sc-514810|sc-13453|sc-134233|

 

Name

4E-BP1 (phospho Thr46) Polyclonal Antibody

Catalogue No.

STJ90162

Reactivity

Human, Mouse, Rat

Specificity

Phospho-4E-BP1 (T46) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T46.

Immunogen

Synthesized phospho-peptide derived from 4E-BP1 (phospho Thr46) at AA range 30-110

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

12/35 kDa

Formulation

4E-BP1 (phospho Thr46) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

4E-BP1 (phospho Thr46) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Eukaryotic translation initiation factor 4E-binding protein 1 antibody, 4E-BP1 antibody, eIF4E-binding protein 1 antibody, Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody, PHAS-I antibody

Database links

Human UniProt/Swiss-Prot:Q13541;Mouse UniPort/Swiss-Prot: Q60876;Rat UniProt/Swiss-Port: Q62622;Human Entrez Gene: 1978;Mouse Entrez Gene: 13685;Rat Entrez Gene: Rn.11161

Protein names

Eukaryotic translation initiation factor 4E-binding protein 1 , 4E-BP1 , eIF4E-binding protein 1 , Phosphorylated heat- and acid-stable protein regulated by insulin 1 , PHAS-I

Protein function

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Protein sequence and domain

The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex. / Belongs to the eIF4E-binding protein family.

Protein post-translational modifications

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E. / Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Research area

All research areas>Signaling Intermediates>4E-BP
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize 4E-BP1 (phospho Thr46) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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