Rabbit Polyclonal Phospho-ABL1-Y412 antibody (AP0303)

$99.00$259.00

Reactivity: Human,Mouse
Applications: IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-ABL1-Y412 antibody (AP0303)

Supplier: ABclonal Inc.

Recommended applications: IHC,IF

Recommended dilution:

IHC 1:50 – 1:100
IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-ABL1-Y412 antibody.

Alternative names:

Check alternative names for the antibody

Expand

ABL antibody; JTK7 antibody; p150 antibody; c-ABL antibody; v-abl antibody; c-ABL1 antibody; bcr/abl antibody;
Abelson murine leukemia viral oncogene homolog 1 antibody|Abelson tyrosine protein kinase 1 antibody|Abl 1 antibody|ABL antibody|ABL proto oncogene 1 non receptor tyrosine kinase antibody|ABL1 antibody|ABL1_HUMAN antibody|bcr/abl antibody|bcr/c abl oncogene protein antibody|c ABL antibody|c abl oncogene 1 non receptor tyrosine kinase antibody|c abl oncogene 1 receptor tyrosine kinase antibody|c ABL1 antibody|JTK7 antibody|p150 antibody|Proto oncogene tyrosine protein kinase ABL1 antibody|Proto-oncogene c-Abl antibody|Tyrosine-protein kinase ABL1 antibody|v abl Abelson murine leukemia viral oncogene homolog 1 antibody|v abl antibody|Phospho anti-c Abl (Y412) antibody (ab47315)

SCBT cat No: sc-887|sc-13076|sc-131|sc-52990|sc-374316|sc-33597|sc-376272|

Name

Rabbit Polyclonal Phospho-ABL1-Y412 antibody

Catalogue No.

AP0303

Reactivity

Human, Mouse

Immunogen

A phospho specific peptide corresponding to residues surrounding Y412 of human ABL1

Host

Rabbit

Applications

IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

122kDa

Formulation

Phospho-ABL1-Y412 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ABL antibody; JTK7 antibody; p150 antibody; c-ABL antibody; v-abl antibody; c-ABL1 antibody; bcr/abl antibody;

Protein names

ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl;

Protein function

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

Protein tissue specificity

Widely expressed.

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.; Contains 1 protein kinase domain.; Contains 1 SH2 domain.; Contains 1 SH3 domain.

Protein post-translational modifications

Acetylated at Lys-711 by EP300 which promotes the cytoplasmic translocation.; Phosphorylation at Tyr-70 by members of the SRC family of kinases disrupts SH3 domain-based autoinhibitory interactions and intermolecular associations, such as that with ABI1, and also enhances kinase activity. Phosphorylation at Tyr-226 and Tyr-393 correlate with increased activity. DNA damage-induced activation of ABL1 requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation at Ser-569 has been attributed to a CDC2-associated kinase and is coupled to cell division (By similarity). Phosphorylation at Ser-618 and Ser-619 by PAK2 increases binding to CRK and reduces binding to ABI1. Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation. Phosphorylated by PRKDC (By similarity).; Polyubiquitinated. Polyubiquitination of ABL1 leads to degradation.

Protein cellular localization

Cytoplasm, cytoskeleton. Nucleus. Mitochondrion . Note=Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity).; Isoform IB: Nucleus membrane; Lipid-anchor. Note=The myristoylated c-ABL protein is reported to be nuclear.

Background

The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/c-Abl/">c-Abl</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-ABL1-Y412 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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