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Rabbit Polyclonal Phospho-ACK (Y284) antibody (STJ91057)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for ACK (phospho Tyr284) Polyclonal Antibody.
Check alternative names for the antibodyExpand
TNK2 antibody, ACK1 antibody,|Acetate kinase 1 antibody|Acetokinase 1 antibody|ACK 1 antibody|ACK antibody|ACK-1 antibody|ACK1 antibody|ACK1_HUMAN antibody|Activated Cdc42 associated kinase 1 antibody|Activated CDC42 kinase 1 antibody|Activated p21cdc42Hs kinase antibody|FLJ44758 antibody|FLJ45547 antibody|p21cdc42Hs antibody|TNK 2 antibody|TNK2 antibody|Tyrosine kinase non receptor 2 antibody|Tyrosine kinase non receptor protein 2 antibody|Tyrosine kinase non-receptor protein 2 antibody|Anti-ACK1 antibody (ab135672)
SCBT cat No: sc-28336|sc-323|
ACK (phospho Tyr284) Polyclonal Antibody
|Catalogue No.|| |
Phospho-ACK (Y284) Polyclonal Antibody detects endogenous levels of ACK protein only when phosphorylated at Y284.
Synthesized phospho-peptide derived from ACK (phospho Tyr284) at AA range 220-300
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;
|Molecular weight|| |
ACK (phospho Tyr284) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
ACK (phospho Tyr284) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Activated CDC42 kinase 1 antibody, ACK-1 antibody, Tyrosine kinase non-receptor protein 2 antibody
|Protein names|| |
Activated CDC42 kinase 1 , ACK-1 , Tyrosine kinase non-receptor protein 2
|Protein function|| |
Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on ‘Tyr-176’. Phosphorylates AR on ‘Tyr-267’ and ‘Tyr-363’ thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on ‘Tyr-287’. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Inhibited by AIM-100 (4-amino-5,6-biaryl-furo[2,3-d]pyrimidine), which suppresses activating phosphorylation at Tyr-284. Repressed by dasatinib.
|Protein tissue specificity|| |
The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages.
|Protein sequence and domain|| |
The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR. / The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation. / The UBA domain binds both poly- and mono-ubiquitin. / Belongs to the protein kinase superfamily. Tyr protein kinase family. / Contains 1 CRIB domain. / Contains 1 protein kinase domain. / Contains 1 SH3 domain. / Contains 1 UBA domain.
|Protein post-translational modifications|| |
Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits. / Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent (By similarity).
|Protein cellular localization|| |
Cell membrane / Nucleus / Endosome / Cell junction > adherens junction / Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side / Cytoplasmic vesicle > clathrin-coated vesicle / Membrane > clathrin-coated pit
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St John’s Laboratory Ltd.
|Product type|| |
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