Rabbit Polyclonal Phospho-ALK (Y1604) antibody (STJ91058)

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Reactivity: Human
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-ALK (Y1604) antibody (STJ91058)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for ALK (phospho Tyr1604) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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ALK antibody,|Alk antibody|ALK tyrosine kinase receptor antibody|ALK/EML4 fusion gene, included antibody|ALK/NPM1 fusion gene, included antibody|ALK_HUMAN antibody|anaplastic lymphoma kinase (Ki-1) antibody|Anaplastic lymphoma kinase antibody|Anaplastic lymphoma kinase Ki1 antibody|anaplastic lymphoma receptor tyrosine kinase antibody|CD 246 antibody|CD246 antibody|CD246 antigen antibody|EC 2.7.10.1 antibody|Ki 1 antibody|Ki1 antibody|mutant anaplastic lymphoma kinase antibody|NBLST 3 antibody|NBLST3 antibody|Tcrz antibody|TFG/ALK antibody|Anti-ALK antibody [5A4] (ab17127)
SCBT cat No: sc-6343|

 

Name

ALK (phospho Tyr1604) Polyclonal Antibody

Catalogue No.

STJ91058

Reactivity

Human

Specificity

Phospho-ALK (Y1604) Polyclonal Antibody detects endogenous levels of ALK protein only when phosphorylated at Y1604.

Immunogen

Synthesized phospho-peptide derived from ALK (phospho Tyr1604) at AA range 1540-1620

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

176 kDa

Formulation

ALK (phospho Tyr1604) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

ALK (phospho Tyr1604) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

ALK tyrosine kinase receptor antibody, Anaplastic lymphoma kinase antibody, CD antigen CD246 antibody

Database links

Human UniProt/Swiss-Prot:Q9UM73;Mouse UniPort/Swiss-Prot: P97793;Human Entrez Gene: 238;Mouse Entrez Gene: 11682;Rat Entrez Gene: Rn.201918

Protein names

ALK tyrosine kinase receptor , Anaplastic lymphoma kinase , CD antigen CD246

Protein function

Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Activated by ligand-binding and subsequent phosphorylation. Inactivated through dephosphorylation by receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) when there is no stimulation by a ligand. Staurosporine, crizotinib and CH5424802 act as inhibitors of ALK kinase activity.

Protein tissue specificity

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

Involvement in disease

Note: A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas.; Note: A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A.; Note: A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.; Neuroblastoma 3 (NBLST3) [MIM:613014]: A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. . Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.; Note: The ALK signaling pathway plays an important role in glioblastoma, the most common malignant brain tumor of adults and one of the most lethal cancers. It regulates both glioblastoma migration and growth.; Note: A chromosomal aberration involving ALK is found in one subject with colorectal cancer. Translocation t(2;2)(p23.1;p23.3). A 5 million base pair tandem duplication generates an in-frame WDCP-ALK gene fusion. .

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. / Contains 1 LDL-receptor class A domain. / Contains 2 MAM domains. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association. / N-glycosylated.

Protein cellular localization

Cell membrane / Single-pass type I membrane protein

Research area

All research areas>Membrane Receptors>ALK
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize ALK (phospho Tyr1604) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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