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Rabbit Polyclonal Phospho-ASK 1 (S83) antibody (STJ90181)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for ASK 1 (phospho Ser83) Polyclonal Antibody.
Check alternative names for the antibodyExpand
MAP3K5 antibody, ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody,|Apoptosis signal regulating kinase 1 antibody|Apoptosis signal-regulating kinase 1 antibody|ASK 1 antibody|ASK-1 antibody|ASK1 antibody|M3K5 antibody|M3K5_HUMAN antibody|MAP/ERK kinase kinase 5 antibody|MAP3K5 antibody|MAPK/ERK kinase kinase 5 antibody|MAPKKK5 antibody|MEK kinase 5 antibody|MEKK 5 antibody|MEKK5 antibody|Mitogen activated protein kinase kinase kinase 5 antibody|Mitogen-activated protein kinase kinase kinase 5 antibody|Anti-ASK1 antibody [EP553Y] (ab45178)
SCBT cat No: sc-390397|sc-5294|sc-390275|sc-7931|sc-49371|sc-6368|sc-166787|sc-374353|sc-68250|sc-293189|
ASK 1 (phospho Ser83) Polyclonal Antibody
|Catalogue No.|| |
Phospho-ASK 1 (S83) Polyclonal Antibody detects endogenous levels of ASK 1 protein only when phosphorylated at S83.
Synthesized phospho-peptide derived from ASK 1 (phospho Ser83) at AA range 20-100
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;
|Molecular weight|| |
ASK 1 (phospho Ser83) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
ASK 1 (phospho Ser83) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Mitogen-activated protein kinase kinase kinase 5 antibody, Apoptosis signal-regulating kinase 1 antibody, ASK-1 antibody, MAPK/ERK kinase kinase 5 antibody, MEK kinase 5 antibody, MEKK 5 antibody
|Protein names|| |
Mitogen-activated protein kinase kinase kinase 5 , Apoptosis signal-regulating kinase 1 , ASK-1 , MAPK/ERK kinase kinase 5 , MEK kinase 5 , MEKK 5
|Protein function|| |
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). Homophilic association of MAP3K5/ASK1 through the C-terminal coiled-coil domains and the heteromeric complex formation of MAP3K5/ASK1 with the reduced form of thioredoxin (TXN), constitutes an inactive form of the kinase . Upon ROS-induced dissociation of TXN from MAP3K5/ASK1, TRAF2 and TRAF6 are reciprocally recruited to MAP3K5/ASK1 and form the active MAP3K5/ASK1 signalosome, in which TRAF2 and TRAF6 appear to facilitate the active configuration of MAP3K5/ASK1 . MAP3K5/ASK1 activity is also regulated through several phosphorylation and dephosphorylation events. Thr-838 is an activating phosphorylation site that is autophosphorylated and phosphorylated by MAP3K6/ASK2 and dephosphorylated by PPP5C . Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2 . Phosphorylation of Ser-966 induces association of MAP3K5/ASK1 with the 14-3-3 family proteins, which suppresses MAP3K5/ASK1 activity . Calcium/calmodulin-activated protein phosphatase calcineurin (PPP3CA) has been shown to directly dephosphorylate this site . SOCS1 binds to ASK1 by recognizing phosphorylation of Tyr-718 and induces MAP3K5/ASK1 degradation in endothelial cells . Also dephosphorylated and activated by PGAM5. Contains an N-terminal autoinhibitory domain. Once activated targeted for proteosomal degradation by RC3H2-mediated ubiquitination .
|Protein tissue specificity|| |
Abundantly expressed in heart and pancreas.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1 and AKT2. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5. / Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination leading to proteasomal degradation.
|Protein cellular localization|| |
Cytoplasm / Endoplasmic reticulum
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St John’s Laboratory Ltd.
|Product type|| |
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