Rabbit Polyclonal Phospho-B23 (T234) antibody (STJ90546)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-B23 (T234) antibody (STJ90546)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for B23 (phospho Thr234) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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NPM1 antibody, NPM antibody,|B23 antibody|MGC104254 antibody|NO38 antibody|NPM antibody|NPM_HUMAN antibody|NPM1 antibody|Nucleolar phosphoprotein B23 antibody|Nucleolar protein NO38 antibody|Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody|Nucleophosmin antibody|nucleophosmin nucleoplasmin family member 1 antibody|Nucleophosmin/nucleoplasmin family member 1 antibody|Numatrin antibody|OTTHUMP00000161024 antibody|OTTHUMP00000161025 antibody|OTTHUMP00000223397 antibody|OTTHUMP00000223398 antibody|Anti-Nucleophosmin antibody [FC82291] (ab10530)
SCBT cat No: sc-47725|sc-70392|sc-65943|sc-6013|sc-271737|sc-56622|sc-32256|sc-5564|sc-53175|

 

Name

B23 (phospho Thr234) Polyclonal Antibody

Catalogue No.

STJ90546

Reactivity

Human, Mouse, Rat

Specificity

Phospho-B23 (T234) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T234.

Immunogen

Synthesized phospho-peptide derived from B23 (phospho Thr234) at AA range 180-260

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

38 kDa

Formulation

B23 (phospho Thr234) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

B23 (phospho Thr234) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Nucleophosmin antibody, NPM antibody, Nucleolar phosphoprotein B23 antibody, Nucleolar protein NO38 antibody, Numatrin antibody

Database links

Human UniProt/Swiss-Prot:P06748;Mouse UniPort/Swiss-Prot: Q61937;Rat UniProt/Swiss-Port: P13084;Human Entrez Gene: 4869;Mouse Entrez Gene: 18148;Rat Entrez Gene: Rn.54537

Protein names

Nucleophosmin , NPM , Nucleolar phosphoprotein B23 , Nucleolar protein NO38 , Numatrin

Protein function

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade .

Involvement in disease

Note: A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. .; Note: A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA. .; Note: A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1. .; Note: Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. .

Protein sequence and domain

Belongs to the nucleoplasmin family.

Protein post-translational modifications

Acetylated at C-terminal lysine residues, thereby increasing affinity to histones. / ADP-ribosylated. / Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi’s sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels. / Sumoylated by ARF.

Protein cellular localization

Nucleus > nucleolus / Nucleus > nucleoplasm / Cytoplasm > cytoskeleton > microtubule organizing center > centrosome

Research area

All research areas>Transcription Regulators>Nucleolar phosphoprotein/nucleophosmin
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize B23 (phospho Thr234) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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