Rabbit Polyclonal Phospho-Bad (S134) antibody (STJ91070)


Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Bad (S134) antibody (STJ91070)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Bad (phospho Ser134) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


BAD antibody, BBC6 antibody, BCL2L8 antibody,|AI325008 antibody|BAD antibody|BAD_HUMAN antibody|BBC 2 antibody|BBC2 antibody|BBC6 antibody|Bcl 2 Antagonist of Cell Death antibody|Bcl 2 Binding Component 6 antibody|BCL X / BCL 2 Binding Protein antibody|BCL X Binding Protein antibody|Bcl XL/Bcl 2 Associated Death Promoter antibody|Bcl-2-binding component 6 antibody|Bcl-2-like protein 8 antibody|Bcl-XL/Bcl-2-associated death promoter antibody|Bcl2 antagonist of cell death antibody|BCL2 antagonist of cell death protein antibody|BCL2 associated agonist of cell death antibody|Bcl2 Associated Death Promoter antibody|BCL2 binding component 6 antibody|BCL2 binding protein antibody|Bcl2 Like 8 Protein antibody|Bcl2-L-8 antibody|BCL2L8 antibody|Proapoptotic BH3 Only Protein antibody|Anti-Bad antibody [Y208] (ab32445)
SCBT cat No: sc-8044|sc-7869|sc-942|sc-166324|sc-33787|sc-32763|sc-48350|sc-137138|sc-365505|sc-365543|



Bad (phospho Ser134) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134.


Synthesized phospho-peptide derived from Bad (phospho Ser134) at AA range 70-150





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;







Molecular weight

28 kDa


Bad (phospho Ser134) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Bad (phospho Ser134) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Bcl2-associated agonist of cell death antibody, BAD antibody, Bcl-2-binding component 6 antibody, Bcl-2-like protein 8 antibody, Bcl2-L-8 antibody, Bcl-xL/Bcl-2-associated death promoter antibody, Bcl2 antagonist of cell death antibody

Database links

Human UniProt/Swiss-Prot:Q92934;Mouse UniPort/Swiss-Prot: Q61337;Rat UniProt/Swiss-Port: O35147;Human Entrez Gene: 572;Mouse Entrez Gene: 12015;Rat Entrez Gene: Rn.36696

Protein names

Bcl2-associated agonist of cell death , BAD , Bcl-2-binding component 6 , Bcl-2-like protein 8 , Bcl2-L-8 , Bcl-xL/Bcl-2-associated death promoter , Bcl2 antagonist of cell death

Protein function

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Protein tissue specificity

Expressed in a wide variety of tissues.

Protein sequence and domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. / Belongs to the Bcl-2 family.

Protein post-translational modifications

Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. / Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.

Protein cellular localization

Mitochondrion outer membrane / Cytoplasm

Research area

All research areas>Tumor Suppressor/Apoptosis>Bad
(View all antibody categories related to Tumor Suppressor/Apoptosis)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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