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Rabbit Polyclonal Phospho-Bcl-6 (S333) antibody (STJ90620)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Bcl-6 (phospho Ser333) Polyclonal Antibody.
Check alternative names for the antibodyExpand
BCL6 antibody, BCL5 antibody, LAZ3 antibody, ZBTB27 antibody, ZNF51 antibody,|B cell CLL/lymphoma 6 antibody|B cell lymphoma 6 protein antibody|B-cell lymphoma 5 protein antibody|B-cell lymphoma 6 protein antibody|BCL 5 antibody|Bcl 6 antibody|BCL-5 antibody|BCL-6 antibody|BCL5 antibody|BCL6 antibody|BCL6_HUMAN antibody|BCL6A antibody|cys his2 zinc finger transcription factor antibody|LAZ 3 antibody|LAZ 3 protein antibody|LAZ3 antibody|Lymphoma associated zinc finger gene on chromosome 3 antibody|Protein LAZ-3 antibody|ZBTB 27 antibody|ZBTB27 antibody|Zinc finger and BTB domain containing protein 27 antibody|Zinc finger and BTB domain-containing protein 27 antibody|Zinc finger protein 51 antibody|zinc finger transcription factor BCL6S antibody|ZNF 51 antibody|ZNF51 antibody|Anti-Bcl6 antibody (ab19011)
SCBT cat No: sc-70414|sc-130916|sc-368|sc-7388|sc-365618|sc-858|sc-56625|
Bcl-6 (phospho Ser333) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Bcl-6 (S333) Polyclonal Antibody detects endogenous levels of Bcl-6 protein only when phosphorylated at S333.
Synthesized phospho-peptide derived from Bcl-6 (phospho Ser333) at AA range 270-350
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:5000;
|Molecular weight|| |
Bcl-6 (phospho Ser333) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Bcl-6 (phospho Ser333) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
B-cell lymphoma 6 protein antibody, BCL-6 antibody, B-cell lymphoma 5 protein antibody, BCL-5 antibody, Protein LAZ-3 antibody, Zinc finger and BTB domain-containing protein 27 antibody, Zinc finger protein 51 antibody
|Protein names|| |
B-cell lymphoma 6 protein , BCL-6 , B-cell lymphoma 5 protein , BCL-5 , Protein LAZ-3 , Zinc finger and BTB domain-containing protein 27 , Zinc finger protein 51
|Protein function|| |
Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5′-TTCCTAGAA-3′ (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
|Protein tissue specificity|| |
Expressed in germinal center T- and B-cells and in primary immature dendritic cells.
|Involvement in disease|| |
Note: Chromosomal aberrations involving BCL6 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL), including diffuse large B-cell lymphoma and follicular lymphoma. Approximately 40% of diffuse large B-cell lymphomas and 5 to 10% of follicular lymphomas are associated with chromosomal translocations that deregulate expression of BCL6 by juxtaposing heterologous promoters to the BCL6 coding domain. Translocation t(3;14)(q27;q32). Translocation t(3;22)(q27;q11) with immunoglobulin gene regions. Translocation t(3;7)(q27;p12) with IKZF1 gene 5’non-coding region. Translocation t(3;6)(q27;p21) with Histone H4. Translocation t(3;16)(q27;p11) with IL21R. Translocation t(3;13)(q27;q14) with LCP1.; Note: A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.; Note: A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF.
|Protein sequence and domain|| |
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2 (PubMed:18212045). Interaction with corepressors through the BTB domain is needed to facilitate the rapid proliferation and survival of GC B-cells but is not involved in the T(FH) formation and BCL6-mediated suppression of T(H)2 and T(H)17 differentiationrequired for GC formation (By similarity). / Contains 1 BTB (POZ) domain. / Contains 6 C2H2-type zinc fingers.
|Protein post-translational modifications|| |
Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. / Polyubiquitinated. Polyubiquitinated by SCF(FBXO11), leading to its degradation by the proteasome. / Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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