Rabbit Polyclonal Phospho-Bcl-x (T47) antibody (STJ90195)


Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Bcl-x (T47) antibody (STJ90195)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Bcl-x (phospho Thr47) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


BCL2L1 antibody, BCL2L antibody, BCLX antibody,|Apoptosis regulator Bcl X antibody|Bcl2-L-1 antibody|BCL2L antibody|Bcl2l1 antibody|BCLX antibody|BclXL antibody|BclXs antibody|DKFZp781P2092 antibody|PPP1R52 antibody|Protein phosphatase 1 regulatory subunit 52 antibody|Anti-Bcl-XL antibody [E18] (ab32370)
SCBT cat No: sc-8274|sc-136132|



Bcl-x (phospho Thr47) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-Bcl-x (T47) Polyclonal Antibody detects endogenous levels of Bcl-x protein only when phosphorylated at T47.


Synthesized phospho-peptide derived from Bcl-x (phospho Thr47) at AA range 30-110





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;







Molecular weight

30 kDa


Bcl-x (phospho Thr47) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Bcl-x (phospho Thr47) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Bcl-2-like protein 1 antibody, Bcl2-L-1 antibody, Apoptosis regulator Bcl-X antibody

Database links

Human UniProt/Swiss-Prot:Q07817;Mouse UniPort/Swiss-Prot: Q64373;Rat UniProt/Swiss-Port: P53563;Human Entrez Gene: 598;Mouse Entrez Gene: 12048;Rat Entrez Gene: Rn.10323

Protein names

Bcl-2-like protein 1 , Bcl2-L-1 , Apoptosis regulator Bcl-X

Protein function

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. / Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F1F0 activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release . / Isoform Bcl-X(S) promotes apoptosis.

Protein tissue specificity

Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

Protein sequence and domain

The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death. / The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. / Belongs to the Bcl-2 family.

Protein post-translational modifications

Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. / Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.

Protein cellular localization

Mitochondrion inner membrane / Mitochondrion outer membrane / Mitochondrion matrix / Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane / Cytoplasm > cytosol / Cytoplasm > cytoskeleton > microtubule organizing center > centrosome / Nucleus membrane

Research area

All research areas>Tumor Suppressor/Apoptosis>Bcl-x
(View all antibody categories related to Tumor Suppressor/Apoptosis)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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