Rabbit Polyclonal Phospho-BLNK (Y96) antibody (STJ90847)


Reactivity: Human, Mouse, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-BLNK (Y96) antibody (STJ90847)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody


BLNK antibody, BASH antibody, SLP65 antibody,|AGM4 antibody|B cell adapter containing SH2 domain protein antibody|B cell adapter containing Src homology 2 domain protein antibody|B cell adaptor containing SH2 domain antibody|B cell linker antibody|B cell linker protein antibody|B cell-specific adaptor protein antibody|B-cell activation antibody|B-cell adapter containing a SH2 domain protein antibody|B-cell adapter containing a Src homology 2 domain protein antibody|B-cell linker protein antibody|BASH antibody|Bca antibody|BLNK antibody|BLNK s antibody|BLNK_HUMAN antibody|Cytoplasmic adapter protein antibody|Ly 57 antibody|Ly57 antibody|Lymphocyte antigen 57 antibody|Lyw 57 antibody|Lyw57 antibody|MGC111051 antibody|SH2 domain-containing leukocyte protein, 65-KD antibody|SLP 65 antibody|SLP-65 antibody|SLP65 antibody|Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody|Src homology 2 domain containing leukocyte protein of 65 kDa antibody|Src homology 2 domain-containing leukocyte protein of 65 kDa antibody|Anti-BLNK antibody [Y491] (ab32418)
SCBT cat No: sc-8003|sc-514780|sc-8382|sc-15345|sc-59459|



BLNK (phospho Tyr96) Polyclonal Antibody

Catalogue No.



Human, Mouse, Monkey


Phospho-BLNK (Y96) Polyclonal Antibody detects endogenous levels of BLNK protein only when phosphorylated at Y96.


Synthesized phospho-peptide derived from BLNK (phospho Tyr96) at AA range 40-120





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;







Molecular weight

50 kDa


BLNK (phospho Tyr96) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


BLNK (phospho Tyr96) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

B-cell linker protein antibody, B-cell adapter containing a SH2 domain protein antibody, B-cell adapter containing a Src homology 2 domain protein antibody, Cytoplasmic adapter protein antibody, Src homology 2 domain-containing leukocyte protein of 65 kDa antibody, SLP-65 antibody

Database links

Human UniProt/Swiss-Prot:Q8WV28;Mouse UniPort/Swiss-Prot: Q9QUN3;Rat UniProt/Swiss-Port: Q4KM52;Human Entrez Gene: 29760;Mouse Entrez Gene: 17060;Rat Entrez Gene: Rn.32684

Protein names

B-cell linker protein , B-cell adapter containing a SH2 domain protein , B-cell adapter containing a Src homology 2 domain protein , Cytoplasmic adapter protein , Src homology 2 domain-containing leukocyte protein of 65 kDa , SLP-65

Protein function

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Protein tissue specificity

Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.

Involvement in disease

Agammaglobulinemia 4, autosomal recessive (AGM4) [MIM:613502]: A primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Contains 1 SH2 domain.

Protein post-translational modifications

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways.

Protein cellular localization

Cytoplasm / Cell membrane

Research area

All research areas>Lymphocyte Signaling>BLNK
(View all antibody categories related to Lymphocyte Signaling)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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