Rabbit Polyclonal Phospho-BRCA1-S988 antibody (AP0318)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-BRCA1-S988 antibody (AP0318)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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IRIS antibody; PSCP antibody; BRCAI antibody; BRCC1 antibody; PNCA4 antibody; RNF53 antibody; BROVCA1 antibody; PPP1R53 antibody;
BRCA 1 antibody|BRCA1 antibody|BRCA1/BRCA2 containing complex subunit 1 antibody|BRCA1/BRCA2-containing complex, subunit 1 antibody|BRCA1_HUMAN antibody|BRCAI antibody|BRCC 1 antibody|BRCC1 antibody|Breast and ovarian cancer susceptibility protein 1 antibody|Breast Cancer 1 antibody|Breast Cancer 1 Early Onset antibody|Breast cancer type 1 susceptibility protein antibody|BROVCA1 antibody|IRIS antibody|PNCA4 antibody|PPP1R53 antibody|Protein phosphatase 1 regulatory subunit 53 antibody|PSCP antibody|RING finger protein 53 antibody|RNF53 antibody|Anti-BRCA1 antibody [MS110] (ab16780)

SCBT cat No: sc-135732|sc-514797|sc-642|sc-641|sc-6954|sc-514640|sc-56030|sc-7867|sc-28234|sc-646|sc-1021|sc-1553|sc-293185|sc-1817|sc-28235|sc-8326|

Name

Rabbit Polyclonal Phospho-BRCA1-S988 antibody

Catalogue No.

AP0318

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S988 of human BRCA1

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

220kDa

Formulation

Phospho-BRCA1-S988 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

IRIS antibody; PSCP antibody; BRCAI antibody; BRCC1 antibody; PNCA4 antibody; RNF53 antibody; BROVCA1 antibody; PPP1R53 antibody;

Protein names

IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53;

Protein function

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).

Protein tissue specificity

Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.

Protein sequence and domain

Contains 2 BRCT domains.; Contains 1 RING-type zinc finger. The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.; The RING-type zinc finger domain interacts with BAP1.

Protein post-translational modifications

Phosphorylation at Ser-308 by AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR. Phosphorylation at Ser-988 by CHEK2 regulates mitotic spindle assembly.; Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.

Protein cellular localization

Nucleus . Chromosome . Cytoplasm . Note=Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by FAM175A and the BRCA1-A complex (PubMed:26778126). Translocated to the cytoplasm during UV-induced apoptosis (PubMed:20160719).; Isoform 3: Cytoplasm.; Isoform 5: Cytoplasm .

Background

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Tumor-SuppressorApoptosis/">Tumor Suppressor/Apoptosis</a>><a href="http://antibodyplus.com/product-tag/BRCA/">BRCA</a><br><a href="http://antibodyplus.com/product-tag/Tumor-SuppressorApoptosis/"&gt; (View all antibody categories related to Tumor Suppressor/Apoptosis)</a>

Note

AntibodyPlus can customize Phospho-BRCA1-S988 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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