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Rabbit Polyclonal Phospho-c-Kit (Y721) antibody (STJ90226)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for c-Kit (phospho Tyr721) Polyclonal Antibody.
Check alternative names for the antibodyExpand
KIT antibody, SCFR antibody,|C Kit antibody|c-Kit antibody|c-Kit Ligand antibody|CD117 antibody|Kit antibody|Kit Ligand antibody|KIT oncogene antibody|KIT proto oncogene receptor tyrosine kinase antibody|KIT_HUMAN antibody|Mast cell growth factor receptor antibody|Mast/stem cell growth factor receptor Kit antibody|MGF antibody|p145 c-kit antibody|PBT antibody|Piebald trait protein antibody|Proto oncogene c Kit antibody|Proto oncogene tyrosine protein kinase Kit antibody|Proto-oncogene c-Kit antibody|SCF Receptor antibody|SCFR antibody|soluble KIT variant 1 antibody|Steel Factor Receptor antibody|Stem cell factor receptor antibody|tyrosine protein kinase Kit antibody|Tyrosine-protein kinase Kit antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody|Anti-c-Kit antibody [YR145] (ab32363)
SCBT cat No: sc-19983|sc-19619|sc-53147|sc-70455|sc-13508|sc-1493|sc-168|sc-17806|sc-365504|sc-393910|sc-5535|sc-1494|
c-Kit (phospho Tyr721) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-c-Kit (Y721) Polyclonal Antibody detects endogenous levels of c-Kit protein only when phosphorylated at Y721.
Synthesized phospho-peptide derived from c-Kit (phospho Tyr721) at AA range 660-740
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
c-Kit (phospho Tyr721) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
c-Kit (phospho Tyr721) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Mast/stem cell growth factor receptor Kit antibody, SCFR antibody, Piebald trait protein antibody, PBT antibody, Proto-oncogene c-Kit antibody, Tyrosine-protein kinase Kit antibody, p145 c-kit antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody, CD antigen CD117 antibody
|Protein names|| |
Mast/stem cell growth factor receptor Kit , SCFR , Piebald trait protein , PBT , Proto-oncogene c-Kit , Tyrosine-protein kinase Kit , p145 c-kit , v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog , CD antigen CD117
|Protein function|| |
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.
|Protein tissue specificity|| |
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
|Involvement in disease|| |
Piebald trait (PBT) [MIM:172800]: Autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Gastrointestinal stromal tumor (GIST) [MIM:606764]: Common mesenchymal neoplasms arising in the gastrointestinal tract, most often in the stomach. They are histologically, immunohistochemically, and genetically different from typical leiomyomas, leiomyosarcomas, and schwannomas. Most GISTs are composed of a fairly uniform population of spindle-shaped cells. Some tumors are dominated by epithelioid cells or contain a mixture of spindle and epithelioid morphologies. Primary GISTs in the gastrointestinal tract commonly metastasize in the omentum and mesenteries, often as multiple nodules. However, primary tumors may also occur outside of the gastrointestinal tract, in other intra-abdominal locations, especially in the omentum and mesentery. . Note: The gene represented in this entry is involved in disease pathogenesis.; Testicular germ cell tumor (TGCT) [MIM:273300]: A common malignancy in males representing 95% of all testicular neoplasms. TGCTs have various pathologic subtypes including: unclassified intratubular germ cell neoplasia, seminoma (including cases with syncytiotrophoblastic cells), spermatocytic seminoma, embryonal carcinoma, yolk sac tumor, choriocarcinoma, and teratoma. Note: The gene represented in this entry may be involved in disease pathogenesis.; Leukemia, acute myelogenous (AML) [MIM:601626]: A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. Note: The gene represented in this entry is involved in disease pathogenesis. Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the kinase domain can result in a constitutively activated kinase.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. / Contains 5 Ig-like C2-type (immunoglobulin-like) domains. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation. / Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
|Protein cellular localization|| |
Cell membrane; Single-pass type I membrane protein / Cell membrane; Single-pass type I membrane protein / Cytoplasm
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St John’s Laboratory Ltd.
|Product type|| |
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