Rabbit Polyclonal Phospho-CaMKIalpha (T177) antibody (STJ91073)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-CaMKIalpha (T177) antibody (STJ91073)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for CaMKIalpha (phospho Thr177) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CAMK1 antibody,|Calcium / calmodulin dependent protein kinase 1 antibody|Calcium / calmodulin dependent protein kinase I antibody|Calcium / calmodulin dependent protein kinase type 1 antibody|Calcium/calmodulin dependent protein kinase 1 antibody|Calcium/calmodulin dependent protein kinase I antibody|Calcium/calmodulin dependent protein kinase type 1 antibody|Calcium/calmodulin-dependent protein kinase type 1 antibody|CaM K1 antibody|CaM KI antibody|CaM kinase 1 alpha antibody|CAM kinase 1 antibody|CaM kinase I alpha antibody|CaM kinase I antibody|CaM-KI antibody|CaMK 1 antibody|CAMK I antibody|CaMK1 alpha antibody|CAMK1 antibody|CAMK1 PEN antibody|CaMKI alpha antibody|CaMKI-alpha antibody|KCC1A_HUMAN antibody|MGC120317 antibody|MGC120318 antibody|Anti-CaMKI antibody [EPR2217Y] (ab68234)
SCBT cat No: sc-1543|sc-377418|sc-33165|sc-137225|sc-1544|

 

Name

CaMKIalpha (phospho Thr177) Polyclonal Antibody

Catalogue No.

STJ91073

Reactivity

Human, Mouse, Rat

Specificity

Phospho-CaMKIalpha (T177) Polyclonal Antibody detects endogenous levels of CaMKIalpha protein only when phosphorylated at T177.

Immunogen

Synthesized phospho-peptide derived from CaMKIalpha (phospho Thr177) at AA range 120-200

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

41 kDa

Formulation

CaMKIalpha (phospho Thr177) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

CaMKIalpha (phospho Thr177) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Calcium/calmodulin-dependent protein kinase type 1 antibody, CaM kinase I antibody, CaM-KI antibody, CaM kinase I alpha antibody, CaMKI-alpha antibody

Database links

Human UniProt/Swiss-Prot:Q14012;Mouse UniPort/Swiss-Prot: Q91YS8;Rat UniProt/Swiss-Port: Q63450;Human Entrez Gene: 8536;Mouse Entrez Gene: 52163;Rat Entrez Gene: Rn.11018

Protein names

Calcium/calmodulin-dependent protein kinase type 1 , CaM kinase I , CaM-KI , CaM kinase I alpha , CaMKI-alpha

Protein function

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca2+ elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on ‘Ser-694’, which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on ‘Ser-91’, ‘Ser-92’, ‘Ser-93’ and ‘Ser-294’. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of ‘Ser-259’ and ‘Ser-498’ in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on ‘Ser-276’ and ‘Ser-295’. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K+ and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. / ATP + a protein = ADP + a phosphoprotein. / Activated by Ca2+/calmodulin. Binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows phosphorylation of Thr-177 within the activation loop by CaMKK1 or CaMKK2. Phosphorylation of Thr-177 results in several fold increase in total activity. Unlike CaMK4, is unable to exhibit autonomous activity after Ca2+/calmodulin activation.

Protein tissue specificity

Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.

Protein sequence and domain

The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. / Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated by CaMKK1 and CaMKK2 on Thr-177. / Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Kinases and Phosphatases>CaMK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize CaMKIalpha (phospho Thr177) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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