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Rabbit Polyclonal Phospho-CaMKIalpha (T177) antibody (STJ91073)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for CaMKIalpha (phospho Thr177) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CAMK1 antibody,|Calcium / calmodulin dependent protein kinase 1 antibody|Calcium / calmodulin dependent protein kinase I antibody|Calcium / calmodulin dependent protein kinase type 1 antibody|Calcium/calmodulin dependent protein kinase 1 antibody|Calcium/calmodulin dependent protein kinase I antibody|Calcium/calmodulin dependent protein kinase type 1 antibody|Calcium/calmodulin-dependent protein kinase type 1 antibody|CaM K1 antibody|CaM KI antibody|CaM kinase 1 alpha antibody|CAM kinase 1 antibody|CaM kinase I alpha antibody|CaM kinase I antibody|CaM-KI antibody|CaMK 1 antibody|CAMK I antibody|CaMK1 alpha antibody|CAMK1 antibody|CAMK1 PEN antibody|CaMKI alpha antibody|CaMKI-alpha antibody|KCC1A_HUMAN antibody|MGC120317 antibody|MGC120318 antibody|Anti-CaMKI antibody [EPR2217Y] (ab68234)
SCBT cat No: sc-1543|sc-377418|sc-33165|sc-137225|sc-1544|
CaMKIalpha (phospho Thr177) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-CaMKIalpha (T177) Polyclonal Antibody detects endogenous levels of CaMKIalpha protein only when phosphorylated at T177.
Synthesized phospho-peptide derived from CaMKIalpha (phospho Thr177) at AA range 120-200
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000;
|Molecular weight|| |
CaMKIalpha (phospho Thr177) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
CaMKIalpha (phospho Thr177) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Calcium/calmodulin-dependent protein kinase type 1 antibody, CaM kinase I antibody, CaM-KI antibody, CaM kinase I alpha antibody, CaMKI-alpha antibody
|Protein names|| |
Calcium/calmodulin-dependent protein kinase type 1 , CaM kinase I , CaM-KI , CaM kinase I alpha , CaMKI-alpha
|Protein function|| |
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca2+ elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on ‘Ser-694’, which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on ‘Ser-91’, ‘Ser-92’, ‘Ser-93’ and ‘Ser-294’. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of ‘Ser-259’ and ‘Ser-498’ in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on ‘Ser-276’ and ‘Ser-295’. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K+ and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. / ATP + a protein = ADP + a phosphoprotein. / Activated by Ca2+/calmodulin. Binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows phosphorylation of Thr-177 within the activation loop by CaMKK1 or CaMKK2. Phosphorylation of Thr-177 results in several fold increase in total activity. Unlike CaMK4, is unable to exhibit autonomous activity after Ca2+/calmodulin activation.
|Protein tissue specificity|| |
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
|Protein sequence and domain|| |
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. / Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated by CaMKK1 and CaMKK2 on Thr-177. / Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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