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Rabbit Polyclonal Phospho-CARM1-S228 antibody (AP0319)
Supplier: ABclonal Inc.
Recommended applications: WB,IF
WB 1:500 – 1:2000
IF 1:100 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit Polyclonal Phospho-CARM1-S228 antibody.
Check alternative names for the antibodyExpand
carm1 antibody|CARM1_HUMAN antibody|Coactivator associated arginine methyltransferase 1 antibody|Coactivator-associated arginine methyltransferase 1 antibody|Histone arginine methyltransferase CARM 1 antibody|Histone arginine methyltransferase CARM1 antibody|Histone-arginine methyltransferase CARM1 antibody|PRMT 4 antibody|PRMT4 antibody|Protein arginine methyltransferase antibody|Protein arginine N methyltransferase 4 antibody|Protein arginine N-methyltransferase 4 antibody|Anti-PRMT4 antibody (ab128851)
SCBT cat No: sc-390656|sc-393381|sc-33176|sc-5421|sc-5418|
Rabbit Polyclonal Phospho-CARM1-S228 antibody
|Catalogue No.|| |
Human, Mouse, Rat
A phospho specific peptide corresponding to residues surrounding S228 of human CARM1
|Recommended dilution|| |
WB 1:500 – 1:2000<br/>IF 1:100 – 1:200
|Molecular weight|| |
Phospho-CARM1-S228 antibody was tube-contained.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/Q86X55">Q86X55</a>
|Protein names|| |
|Protein function|| |
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
|Protein tissue specificity|| |
Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
|Protein sequence and domain|| |
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.; Contains 1 SAM-dependent MTase PRMT-type domain.
|Protein post-translational modifications|| |
Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing (By similarity).; Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
|Protein cellular localization|| |
Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/CARM/">CARM</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
AntibodyPlus can customize Phospho-CARM1-S228 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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