Rabbit Polyclonal Phospho-Casein Kinase IIalpha (Y255) antibody (STJ90551)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Casein Kinase IIalpha (Y255) antibody (STJ90551)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Casein Kinase IIalpha (phospho Tyr255) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CSNK2A1 antibody, CK2A1 antibody,|Casein kinase 2 alpha 1 polypeptide antibody|Casein kinase II alpha 1 antibody|Casein kinase II alpha 1 subunit antibody|Casein kinase II alpha subunit antibody|Casein kinase II subunit alpha antibody|CK II alpha antibody|CK II antibody|CK2 alpha antibody|CK2 catalytic subunit alpha antibody|CK2A1 antibody|CKII antibody|CKIIalpha antibody|CSK21_HUMAN antibody|CSNK2A1 antibody|Anti-CKII alpha antibody [8E5] – ChIP Grade (ab70774)
SCBT cat No: sc-12738|sc-6479|sc-365762|sc-365787|sc-373894|sc-365763|sc-9030|sc-6481|

 

Name

Casein Kinase IIalpha (phospho Tyr255) Polyclonal Antibody

Catalogue No.

STJ90551

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Casein Kinase IIalpha (Y255) Polyclonal Antibody detects endogenous levels of Casein Kinase IIalpha protein only when phosphorylated at Y255.

Immunogen

Synthesized phospho-peptide derived from Casein Kinase IIalpha (phospho Tyr255) at AA range 190-270

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

45 kDa

Formulation

Casein Kinase IIalpha (phospho Tyr255) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Casein Kinase IIalpha (phospho Tyr255) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Casein kinase II subunit alpha antibody, CK II alpha antibody,

Database links

Human UniProt/Swiss-Prot:P68400;Mouse UniPort/Swiss-Prot: Q60737;Rat UniProt/Swiss-Port: P19139;Human Entrez Gene: 1457;Mouse Entrez Gene: 12995;Rat Entrez Gene: Rn.203090

Protein names

Casein kinase II subunit alpha , CK II alpha ,

Protein function

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating ‘Ser-392’ of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. Phosphorylates PML at ‘Ser-565’ and primes it for ubiquitin-mediated degradation. Plays an important role in the circadian clock function by phosphorylating ARNTL/BMAL1 at ‘Ser-90’ which is pivotal for its interaction with CLOCK and which controls CLOCK nuclear entry . Phosphorylates CCAR2 at ‘Thr-454’ in gastric carcinoma tissue . / ATP + a protein = ADP + a phosphoprotein. / Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization.

Protein tissue specificity

Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level).

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated at Thr-344, Thr-360, Ser-362 and Ser-370 by CDK1 in prophase and metaphase and dephosphorylated during anaphase. Phosphorylation does not directly affect casein kinase 2 activity, but may contribute to its regulation by forming binding sites for interacting proteins and/or targeting it to different compartments.

Protein cellular localization

Nucleus

Research area

All research areas>Kinases and Phosphatases>casein kinase II alpha
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Casein Kinase IIalpha (phospho Tyr255) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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