Rabbit Polyclonal Phospho-Caspase-8 (S347) antibody (STJ90205)


Reactivity: Human, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-Caspase-8 (S347) antibody (STJ90205)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Caspase-8 (phospho Ser347) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


CASP8 antibody, MCH5 antibody,|ALPS2B antibody|Caspase-8 subunit p10 antibody|CED 3 antibody|FADD Like ICE antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|FLJ17672 antibody|ICE-like apoptotic protease 5 antibody|MACH alpha 1/2/3 protein antibody|MACH antibody|MACH beta 1/2/3/4 protein antibody|MCH5 antibody|MGC78473 antibody|MORT1 associated ced 3 homolog antibody|MORT1-associated CED-3 homolog antibody|OTTHUMP00000163717 antibody|OTTHUMP00000163720 antibody|OTTHUMP00000163724 antibody|OTTHUMP00000163725 antibody|OTTHUMP00000165062 antibody|OTTHUMP00000165063 antibody|OTTHUMP00000165064 antibody|OTTHUMP00000206552 antibody|OTTHUMP00000206582 antibody|Anti-Caspase-8 antibody (ab25901)
SCBT cat No: sc-81656|sc-81657|sc-70501|sc-73526|sc-70502|sc-81661|sc-81662|sc-56070|sc-6133|sc-56072|sc-56071|sc-6136|sc-393776|sc-5263|sc-166320|sc-166596|sc-7890|sc-6134|



Caspase-8 (phospho Ser347) Polyclonal Antibody

Catalogue No.



Human, Rat


Phospho-Caspase-8 (S347) Polyclonal Antibody detects endogenous levels of Caspase-8 protein only when phosphorylated at S347.


Synthesized phospho-peptide derived from Caspase-8 (phospho Ser347) at AA range 290-370





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;







Molecular weight

55 kDa


Caspase-8 (phospho Ser347) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Caspase-8 (phospho Ser347) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Caspase-8 antibody, CASP-8 antibody, Apoptotic cysteine protease antibody, Apoptotic protease Mch-5 antibody, CAP4 antibody, FADD-homologous ICE/ced-3-like protease antibody, FADD-like ICE antibody, FLICE antibody, ICE-like apoptotic protease 5 antibody, MORT1-associated ced-3 homolog antibody, MACH antibody

Database links

Human UniProt/Swiss-Prot:Q14790;Mouse UniPort/Swiss-Prot: O89110;Rat UniProt/Swiss-Port: Q9JHX4;Human Entrez Gene: 841;Mouse Entrez Gene: 12370;Rat Entrez Gene: Rn.54474

Protein names

Caspase-8 , CASP-8 , Apoptotic cysteine protease , Apoptotic protease Mch-5 , CAP4 , FADD-homologous ICE/ced-3-like protease , FADD-like ICE , FLICE , ICE-like apoptotic protease 5 , MORT1-associated ced-3 homolog , MACH

Protein function

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-, -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. / Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-, -(Gly/Ser/Ala). / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.

Protein tissue specificity

Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

Involvement in disease

Caspase-8 deficiency (CASP8D) [MIM:607271]: Disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. / Belongs to the peptidase C14A family. / Contains 2 DED (death effector) domains.

Protein post-translational modifications

Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events. / Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.

Protein cellular localization


Research area

All research areas>Tumor Suppressor/Apoptosis>Caspase
(View all antibody categories related to Tumor Suppressor/Apoptosis)


AntibodyPlus can customize Caspase-8 (phospho Ser347) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:

Welcome to AntibodyPlus!