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Rabbit Polyclonal Phospho-Caspase-9 (T125) antibody (STJ90758)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Caspase-9 (phospho Thr125) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CASP9 antibody, MCH6 antibody,|APAF-3 antibody|Apoptotic protease Mch-6 antibody|Caspase 9 Dominant Negative antibody|Caspase 9c antibody|Caspase-9 antibody|Caspase-9 subunit p10 antibody|ICE LAP6 antibody|ICE like apoptotic protease 6 antibody|ICE-LAP6 antibody|ICE-like apoptotic protease 6 antibody|MCH6 antibody|PPP1R56 antibody|protein phosphatase 1, regulatory subunit 56 antibody|RNCASP9 antibody|Anti-Caspase-9 antibody [E23] (ab32539)
SCBT cat No: sc-81650|sc-56073|sc-70505|sc-70506|sc-70507|sc-56076|sc-81663|sc-81589|sc-73548|sc-56077|sc-8297|sc-17784|sc-7885|sc-133109|sc-8355|sc-374489|sc-373777|
Caspase-9 (phospho Thr125) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Caspase-9 (T125) Polyclonal Antibody detects endogenous levels of Caspase-9 protein only when phosphorylated at T125.
Synthesized phospho-peptide derived from Caspase-9 (phospho Thr125) at AA range 60-140
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
Caspase-9 (phospho Thr125) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Caspase-9 (phospho Thr125) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Caspase-9 antibody, CASP-9 antibody, Apoptotic protease Mch-6 antibody, Apoptotic protease-activating factor 3 antibody, APAF-3 antibody, ICE-like apoptotic protease 6 antibody, ICE-LAP6 antibody
|Protein names|| |
Caspase-9 , CASP-9 , Apoptotic protease Mch-6 , Apoptotic protease-activating factor 3 , APAF-3 , ICE-like apoptotic protease 6 , ICE-LAP6
|Protein function|| |
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). / Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. / Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-, -Xaa. / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.
|Protein tissue specificity|| |
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
|Protein sequence and domain|| |
Belongs to the peptidase C14A family. / Contains 1 CARD domain.
|Protein post-translational modifications|| |
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. / Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
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St John’s Laboratory Ltd.
|Product type|| |
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