Rabbit Polyclonal Phospho-CBL-Y700 antibody (AP0292)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CBL-Y700 antibody (AP0292)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:1000
IHC 1:50 – 1:100
IF 1:20 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CBL2 antibody; NSLL antibody; C-CBL antibody; RNF55 antibody; FRA11B
4732447J05Rik antibody|C CBL antibody|Cas Br M (murine) ecotropic retroviral transforming sequence antibody|Casitas B lineage lymphoma proto oncogene antibody|Casitas B-lineage lymphoma proto-oncogene antibody|CBL 2 antibody|cbl antibody|CBL_HUMAN antibody|CBL2 antibody|E3 ubiquitin protein ligase CBL antibody|E3 ubiquitin-protein ligase CBL antibody|Oncogene CBL2 antibody|Proto oncogene c CBL antibody|Proto-oncogene c-CBL antibody|RGD1561386 antibody|RING finger protein 55 antibody|RNF55 antibody|Signal transduction protein CBL antibody|Phospho anti-CBL (Y774) antibody [E160] (ab32446)

SCBT cat No: sc-1651|sc-170|sc-393543|sc-398282|sc-8372|sc-390648|sc-390649|

Name

Rabbit Polyclonal Phospho-CBL-Y700 antibody

Catalogue No.

AP0292

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding Y700 of human CBL

Host

Rabbit

Applications

WB, IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

120kDa

Formulation

Phospho-CBL-Y700 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CBL2 antibody; NSLL antibody; C-CBL antibody; RNF55 antibody; FRA11B

Protein names

CBL2; NSLL; C-CBL; RNF55; FRA11B

Protein function

Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, EGFR, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

Protein sequence and domain

Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.; Contains 1 RING-type zinc finger.; Contains 1 UBA domain. The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.; The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

Protein post-translational modifications

Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK.; Ubiquitinated, leading to its degradation via the proteasome.

Protein cellular localization

Cytoplasm. Cell membrane. Note=Colocalizes with FGFR2 in lipid rafts at the cell membrane.

Background

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Signaling-Intermediates/">Signaling Intermediates</a>><a href="http://antibodyplus.com/product-tag/Cbl/">Cbl</a><br><a href="http://antibodyplus.com/product-tag/Signaling-Intermediates/"&gt; (View all antibody categories related to Signaling Intermediates)</a>

Note

AntibodyPlus can customize Phospho-CBL-Y700 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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