No products in the cart.
Rabbit Polyclonal Phospho-CCNB1-S147 antibody (AP0287)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC,IF
WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:100 – 1:200
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit Polyclonal Phospho-CCNB1-S147 antibody.
Check alternative names for the antibodyExpand
CCNB 1 antibody|CCNB antibody|ccnb1 antibody|CCNB1_HUMAN antibody|Cyclin B1 antibody|G2 mitotic specific cyclin B1 antibody|G2/mitotic-specific cyclin-B1 antibody|Anti-Cyclin B1 antibody [V152] (ab72)
SCBT cat No: sc-245|
Rabbit Polyclonal Phospho-CCNB1-S147 antibody
|Catalogue No.|| |
A phospho specific peptide corresponding to residues surrounding S147 of human CCNB1
WB, IHC, IF
|Recommended dilution|| |
WB 1:500 – 1:2000<br/>IHC 1:50 – 1:100<br/>IF 1:100 – 1:200
|Molecular weight|| |
Phospho-CCNB1-S147 antibody was tube-contained.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/P14635">P14635</a>
|Protein names|| |
|Protein function|| |
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
|Protein sequence and domain|| |
Belongs to the cyclin family. Cyclin AB subfamily.
|Protein post-translational modifications|| |
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.; Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
|Protein cellular localization|| |
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Cell-Cycle-Proteins/">Cell Cycle Proteins</a>><a href="http://antibodyplus.com/product-tag/Cyclin/">Cyclin</a><br><a href="http://antibodyplus.com/product-tag/Cell-Cycle-Proteins/"> (View all antibody categories related to Cell Cycle Proteins)</a>
AntibodyPlus can customize Phospho-CCNB1-S147 antibody according to your requirement, including bulk product size,etc. Please contact firstname.lastname@example.org. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
Displaying 1 - 1 of 1
Antibody customer reviews & validation data - 237
Validation data ID: 237
Submission date: 2017-10-09
Rabbit Polyclonal Phospho-CCNB1- S147 antibody (AP0287)
Product catalog No.
St John's Laboratory
Antibody specificity rating
Not work in this case
Antibody overall rating
Not work in this case
The Ab staining does not match with total cyclin B localization. In other words, even though cyclin B localized in the cytoplasm before entering M phase, the Ab stained nuclear protein (probably non-specific protein).
There is no extra validation for this product yet.
Check other extra validated antibodies below: