Rabbit Polyclonal Phospho-Cdc25A (S124) antibody (STJ90511)

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Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Cdc25A (S124) antibody (STJ90511)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cdc25A (phospho Ser124) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CDC25A antibody,|Cdc 25a antibody|CDC25A antibody|CDC25A2 antibody|CDC25A2 CAG isoform antibody|Cell division cycle 25 homolog A (S. pombe) antibody|Cell division cycle 25A antibody|Cell division cycle 25A isoform a antibody|Cell division cycle 25A isoform b antibody|D9Ertd393e antibody|Dual specificity phosphatase Cdc25A antibody|M phase inducer phosphatase 1 antibody|M-phase inducer phosphatase 1 antibody|MGC115549 antibody|MPIP1_HUMAN antibody|Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)
SCBT cat No: sc-97|sc-70823|sc-70824|sc-56263|sc-56264|sc-65503|sc-65505|sc-7389|sc-7157|sc-6947|sc-56265|sc-70825|sc-326|

 

Name

Cdc25A (phospho Ser124) Polyclonal Antibody

Catalogue No.

STJ90511

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Cdc25A (S124) Polyclonal Antibody detects endogenous levels of Cdc25A protein only when phosphorylated at S124.

Immunogen

Synthesized phospho-peptide derived from Cdc25A (phospho Ser124) at AA range 60-140

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

70 kDa

Formulation

Cdc25A (phospho Ser124) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cdc25A (phospho Ser124) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

M-phase inducer phosphatase 1 antibody, Dual specificity phosphatase Cdc25A antibody

Database links

Human UniProt/Swiss-Prot:P30304;Mouse UniPort/Swiss-Prot: P48964;Rat UniProt/Swiss-Port: P48965;Human Entrez Gene: 993;Mouse Entrez Gene: 12530;Rat Entrez Gene: Rn.11390

Protein names

M-phase inducer phosphatase 1 , Dual specificity phosphatase Cdc25A

Protein function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. / Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. / Stimulated by B-type cyclins. Stimulated by PIM1-mediated phosphorylation.

Protein sequence and domain

The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction. / Belongs to the MPI phosphatase family. / Contains 1 rhodanese domain.

Protein post-translational modifications

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation. / Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

Research area

All research areas>Cell Cycle Proteins>Cdc
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Cdc25A (phospho Ser124) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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