Rabbit Polyclonal Phospho-CDC25A-S76 antibody (AP0321)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CDC25A-S76 antibody (AP0321)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-CDC25A-S76 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CDC25A2 antibody;
Cdc 25a antibody|CDC25A antibody|CDC25A2 antibody|CDC25A2 CAG isoform antibody|Cell division cycle 25 homolog A (S. pombe) antibody|Cell division cycle 25A antibody|Cell division cycle 25A isoform a antibody|Cell division cycle 25A isoform b antibody|D9Ertd393e antibody|Dual specificity phosphatase Cdc25A antibody|M phase inducer phosphatase 1 antibody|M-phase inducer phosphatase 1 antibody|MGC115549 antibody|MPIP1_HUMAN antibody|Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

SCBT cat No: sc-97|sc-70823|sc-70824|sc-56263|sc-56264|sc-65503|sc-65505|sc-7389|sc-7157|sc-6947|sc-56265|sc-70825|sc-326|

Name

Rabbit Polyclonal Phospho-CDC25A-S76 antibody

Catalogue No.

AP0321

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S76 of human CDC25A

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

70kDa

Formulation

Phospho-CDC25A-S76 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDC25A2 antibody;

Protein names

CDC25A2;

Protein function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Protein sequence and domain

Belongs to the MPI phosphatase family.; Contains 1 rhodanese domain. The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

Protein post-translational modifications

Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.; Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.

Background

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Cell-Cycle-Proteins/">Cell Cycle Proteins</a>><a href="http://antibodyplus.com/product-tag/Cdc/">Cdc</a><br><a href="http://antibodyplus.com/product-tag/Cell-Cycle-Proteins/"&gt; (View all antibody categories related to Cell Cycle Proteins)</a>

Note

AntibodyPlus can customize Phospho-CDC25A-S76 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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