Rabbit Polyclonal Phospho-Cdc25B (S323) antibody (STJ90217)


Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Cdc25B (S323) antibody (STJ90217)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cdc25B (phospho Ser323) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


CDC25B antibody, CDC25HU2 antibody,|AI604853 antibody|Cdc 25B antibody|Cdc25b antibody|CDC25HU2 antibody|Cdc25m2 antibody|Cell division cycle 25 homolog B antibody|Cell division cycle 25B antibody|Cell division cycle 25B isoform 1 antibody|Cell division cycle 25B isoform 2 antibody|Cell division cycle 25B isoform 3 antibody|Cell division cycle 25B isoform 4 antibody|Cell division cycle 25B isoform 5 antibody|Dual specificity phosphatase Cdc25B antibody|EC antibody|M phase inducer phosphatase 2 antibody|M-phase inducer phosphatase 2 antibody|MPIP2_HUMAN antibody|Phospho anti-Cdc25B (S323) antibody (ab53103)
SCBT cat No: sc-70825|sc-326|sc-56266|sc-65504|sc-6948|sc-5619|sc-56357|sc-327|



Cdc25B (phospho Ser323) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-Cdc25B (S323) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S323.


Synthesized phospho-peptide derived from Cdc25B (phospho Ser323) at AA range 260-340





Recommended dilution

WB 1:500-1:2000; ELISA 1:20000;







Molecular weight

70 kDa


Cdc25B (phospho Ser323) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Cdc25B (phospho Ser323) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

M-phase inducer phosphatase 2 antibody, Dual specificity phosphatase Cdc25B antibody

Database links

Human UniProt/Swiss-Prot:P30305;Mouse UniPort/Swiss-Prot: P30306;Rat UniProt/Swiss-Port: P48966;Human Entrez Gene: 994;Mouse Entrez Gene: 12531;Rat Entrez Gene: Rn.11312

Protein names

M-phase inducer phosphatase 2 , Dual specificity phosphatase Cdc25B

Protein function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. / Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. / Stimulated by B-type cyclins.

Protein sequence and domain

Belongs to the MPI phosphatase family. / Contains 1 rhodanese domain.

Protein post-translational modifications

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.

Protein cellular localization

Cytoplasm > cytoskeleton > microtubule organizing center > centrosome / Cytoplasm > cytoskeleton > spindle pole

Research area

All research areas>Cell Cycle Proteins>Cdc
(View all antibody categories related to Cell Cycle Proteins)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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