Rabbit Polyclonal Phospho-CDC25C-S216 antibody (AP0323)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CDC25C-S216 antibody (AP0323)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CDC25 antibody; PPP1R60 antibody;
CDC 25 antibody|Cdc 25C antibody|CDC25 antibody|CDC25C antibody|Cell division cycle 25 homolog C antibody|Cell division cycle 25C antibody|Cell division cycle 25C protein antibody|Dual specificity phosphatase Cdc25C antibody|M phase inducer phosphatase 3 antibody|M-phase inducer phosphatase 3 antibody|Mitosis inducer CDC25 antibody|MPIP3 antibody|MPIP3_HUMAN antibody|Phosphotyrosine phosphatase antibody|PPP1R60 antibody|protein phosphatase 1, regulatory subunit 60 antibody|Anti-Cdc25C antibody [E302] (ab32444)

SCBT cat No: sc-327|sc-55513|sc-5620|sc-13138|sc-56269|sc-377338|

Name

Rabbit Polyclonal Phospho-CDC25C-S216 antibody

Catalogue No.

AP0323

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S216 of human CDC25C

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

80kDa

Formulation

Phospho-CDC25C-S216 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDC25 antibody; PPP1R60 antibody;

Protein names

CDC25; PPP1R60;

Protein function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Protein sequence and domain

Belongs to the MPI phosphatase family.; Contains 1 rhodanese domain.

Protein post-translational modifications

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Protein cellular localization

Nucleus .

Background

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Cell-Cycle-Proteins/">Cell Cycle Proteins</a>><a href="http://antibodyplus.com/product-tag/Cdc/">Cdc</a><br><a href="http://antibodyplus.com/product-tag/Cell-Cycle-Proteins/"&gt; (View all antibody categories related to Cell Cycle Proteins)</a>

Note

AntibodyPlus can customize Phospho-CDC25C-S216 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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